Urethral Damage
Urethral Damage - Related Videos
05:09
Urethral Stricture Induction Followed by Buccal Mucosa Graft Urethroplasty in a Rat Model
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Cited by 3 •
2023
In the present protocol, a urethral stricture induction was developed in Wistar rats, followed by urethral reconstruction with a buccal mucosa graft. A retrograde urethrogram and laser Doppler assessment were performed, validating urethral reconstruction (after stricture formation) and graft placement.
07:42
Nerve-sparing Mid-urethral Obstruction (NeMO) in Female Small Rodents
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Cited by 7 •
2017
Traditional modeling of partial bladder outlet obstruction in rodents is fraught with animal mortality. A denervation injury from dissection around the proximal urethra and bladder neck is also of major concern. We developed and evaluated a safe and reliable mid-urethral obstruction model, avoiding the shortcomings of the traditional model.
06:19
Comet Assay for DNA Damage Detection in Cells
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2025
The video demonstrates the comet assay for detecting DNA damage following exposure to harmful agents. Under alkaline conditions, the DNA unwinds and denatures, and upon electrophoresis, the undamaged and damaged DNA migrate differently in the agarose gel, forming a comet-shaped structure. The slowly migrating undamaged DNA forms the circular comet head, while the rapidly migrating damaged DNA fragments form the elongated comet tail.
04:09
Assessing DNA Damage Foci: To Identify Radiation-Induced DNA Damage Foci in Cancer Cell Line Using Immunofluorescence Staining
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2023
This video describes a protocol to detect radiation-induced DNA repair foci in the colon cancer cell lines using immunofluorescence staining. DNA repair proteins get activated at the DNA damage site; therefore, we can detect them using specific antibodies and visualize them under a fluorescence microscope.
10:00
Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay
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Cited by 21 •
2012
The comet assay is an efficient way of detecting single- and double-strand breaks, including alkali-labile sites and DNA-DNA/DNA-protein cross-links on the DNA in all cells including hippocampal neurons. The method takes advantage of the differential migration of DNA in an electric field due to differences in amount of DNA damage.
FAQs
These causes can disrupt the urethral lining and surrounding tissue. The resulting bleeding and swelling may be followed by scar formation during healing. When scar tissue narrows the channel, urinary flow can become impaired. Recognizing this sequence helps clinicians connect an initiating event or disease with later obstruction and other urinary dysfunction.
Obstruction may recur when healing produces scar tissue that narrows the urethral channel again. The narrowing can interfere with urine passage and contribute to long-term urinary dysfunction. This mechanism explains why evaluation must consider not only the initial injury but also the extent of scarring and the possibility of persistent or returning flow impairment.
Cause and extent provide important context for choosing an appropriate intervention. Clinical findings, imaging, or endoscopy can help characterize the affected area and the degree of disruption or narrowing. That information supports decisions among catheterization, dilation, urethrotomy, or reconstructive surgery rather than treating every case through the same approach.
Assessment may begin with a clinical examination and then use imaging or endoscopy to investigate the urethra more directly. These approaches help identify the presence and extent of injury, swelling, or scar-related narrowing. The resulting information supports treatment planning and helps clinicians address risks of obstruction and ongoing urinary dysfunction.
Management options include catheterization, dilation, urethrotomy, and reconstructive surgery. The choice depends on the clinical assessment, including the cause and extent of the damage. These interventions are used within a broader plan to restore or support urinary passage, address narrowing, and reduce complications associated with recurrent obstruction or lasting dysfunction.
Prompt recognition and appropriate management can help limit the consequences of bleeding, swelling, scar formation, and narrowing. In medical practice, attention to the underlying cause and the damaged area supports selection of suitable treatment. This approach aims to reduce recurrent obstruction and protect against long-term urinary dysfunction, including problems affecting continence.