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Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay

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10:00 min

December 19, 2012

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10:00 min
December 19, 2012

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Summary

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The comet assay is an efficient way of detecting single- and double-strand breaks, including alkali-labile sites and DNA-DNA/DNA-protein cross-links on the DNA in all cells including hippocampal neurons. The method takes advantage of the differential migration of DNA in an electric field due to differences in amount of DNA damage.

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