Fluorescein Tracking
Fluorescein Tracking - Related Videos
07:35
Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
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Cited by 8 •
2011
This protocol delineates a way to label and trace the fate of small groups of cells zebrafish embryos using UV-uncaging of caged fluorescein, followed by whole mount immunolabeling to amplify the signal from the uncaged fluorescein.
02:01
Fluorescein Staining in an Autoimmune Dry Eye Rat Model to Examine Corneal Damage
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2025
This video demonstrates the staining of the damaged cornea using fluorescein dye to evaluate the extent of autoimmune dry eye disease in a rat model. The green fluorescence spots of the stained collagen fibers indicate corneal epithelial damaged cells.
05:55
Multiple Object Tracking
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2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University In a staggeringly complex and engaging world, it is crucial to selectively process some stimuli at the expense of others. Experimental psychologists call this ability attention. Specifically, visual attention refers to the ability to selectively process aspects of a visual scene. Many paradigms used to study visual attention involve brief, punctuated, and repeated trials. However, everyday situations often place sustained demands...
06:19
Eye Tracking in Cognitive Experiments
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2023
Eye tracking as the name suggests involves tracking of eye-movements. It is a non-invasive, sensitive tool that quantifies and measures eye-movements to describe an individuals' cognitive state. An eye-movement between two fixation points is called a saccade, which is one of the fastest motor movements in our body. By observing the profiles of these eye movements, scientists can better understand neural deficits in patients with cognitive impairments. In this video, we will first look at an...
09:03
Eye Tracking Young Children with Autism
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Cited by 93 •
2012
Eye tracking has long been used to study gaze patterns in typically-developing individuals, but recent technological advancements have made its use with clinical populations, including autism, more feasible. While eye-tracking young children with autism can offer insight into early symptom manifestations, it involves methodological challenges. Suggestions for best practices are provided.
FAQs
Fluorescein absorbs light at shorter wavelengths and then emits green fluorescence at longer wavelengths. This shift allows an imaging system to distinguish emitted signal from the illumination used to excite the dye. By recording where the green signal appears and how its position changes, researchers can visualize labeled material within a biological sample and follow transport over time.
The labeling strategy determines which transport process the observed signal represents. Depending on what receives the fluorescein label and how the experiment is designed, measurements may reflect diffusion through neural tissue, axonal transport, tracer uptake, or movement across cellular barriers. Therefore, the same fluorescent readout can address different biological questions, but its interpretation depends on the experimental design.
Fluorescence microscopy makes the labeled tracer distinguishable from surrounding biological material by detecting its emitted green signal. Imaging can reveal the tracer's location within neural tissue and document changes in position that indicate movement. This capability supports examination of transport pathways and tissue organization when the underlying process would otherwise be difficult to observe directly.
Changes in signal location provide evidence about where a labeled molecule or tracer is distributed and whether it moves through the sample. In neuroscience, spatial patterns can help indicate diffusion through tissue, uptake by cells, or transport along axons, depending on the labeling strategy. Comparing these patterns can reveal differences among pathways or experimental models.
A typical workflow begins by associating fluorescein with the molecule or tracer of interest, placing the labeled material in the biological sample, and imaging the resulting fluorescence with microscopy or a related system. Researchers then examine the signal's location and movement, using the recorded pattern to evaluate transport, distribution, or pathway differences within the chosen experimental design.
Neuroscientists can use this approach when they need to examine neural connectivity, tissue organization, or transport dynamics. Labeled tracers may be selected to monitor diffusion through neural tissue, axonal transport, tracer uptake, or movement across cellular barriers. The method is especially useful for comparing how labeled material follows different pathways or changes across experimental models.
Researchers can compare the location, distribution, and apparent movement of fluorescein-labeled material across pathways or models. Differences in the recorded fluorescence pattern may indicate altered transport, uptake, diffusion, or barrier passage, provided the labeling strategy and imaging design are comparable. These measurements can therefore connect visible tracer behavior with changes in neural tissue organization or transport dynamics.