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Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
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Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran

Article DOI: 10.3791/2672 • 07:36 min • April 28th, 2011
April 28th, 2011 •
1, 2, 2, 1
1Department of Biological Sciences, Vanderbilt University, 2Department of Chemical and Systems Biology, Stanford University

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Summary
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This protocol delineates a way to label and trace the fate of small groups of cells zebrafish embryos using UV-uncaging of caged fluorescein, followed by whole mount immunolabeling to amplify the signal from the uncaged fluorescein.

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Lineage Labeling Zebrafish Cells Laser Uncagable Fluorescein Dextran Developmental Biology Cell Types Lineage Labeling Methods DiI Labeling Traceable Enzymes Zebrafish Labs Photoconvertable Fluorescent Proteins Eos Kaede Ultraviolet Light Transgenesis Dye-mediated Lineage Labeling Methods
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