Intracardiac Perfusion Fixation
Intracardiac Perfusion Fixation - Related Videos
08:53
Whole Animal Perfusion Fixation for Rodents
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Cited by 531 •
2012
Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.
07:19
Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation
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Cited by 3 •
2021
Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.
05:53
Labeling of Blood Vessels in the Teleost Brain and Pituitary Using Cardiac Perfusion with a DiI-fixative
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Cited by 17 •
2019
The article describes a quick protocol for labeling blood vessels in a teleost fish by cardiac perfusion of DiI diluted in fixative, using medaka (Oryzias latipes) as a model and focusing on brain and pituitary tissue.
03:18
Stimulation of the Intracardiac Nervous System to Assess Susceptibility to Arrhythmia
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2025
Source: Jungen, C., et al. Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System. J. Vis. Exp. (2018).The video demonstrates an ex vivo technique for stimulating the intracardiac nervous system to assess the heart’s susceptibility to arrhythmia. Using a perfusion apparatus, an isolated mouse heart is perfused with an oxygenated buffer. The intracardiac nervous system is then stimulated at a high frequency to induce arrhythmias.
07:32
Porcine Normothermic Isolated Liver Perfusion
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Cited by 3 •
2023
The porcine model of liver normothermic machine perfusion (NMP), described here, can be successfully used to study NMP as a preservation strategy, a tool for viability assessment, and a platform for organ repair. It holds a high translational value, however it is technically challenging and labor-intensive.
FAQs
Buffered saline is delivered first to clear circulating blood before the fixative enters the vascular system. This sequence allows the subsequent fixative to travel through cerebral vessels without the blood remaining in circulation. For neuroscience specimens, that staged exchange supports more consistent preservation of brain structure and molecular features for later tissue analysis.
The cannula places fluid into the left ventricle, allowing it to enter the cardiovascular circulation rather than being applied directly to the brain surface. Fixative can therefore move through cerebral vessels, while fluid exits from the right side of the heart. This route uses vascular access to expose neural tissue throughout the brain to preservation conditions.
Preservation must support several levels of observation: brain anatomy, the organization of cells within neural tissue, and molecular features that can be examined after fixation. Maintaining these features is important because neuroscience studies may need to relate microscopic structure to disease-related changes. The resulting specimen is therefore suited to analyses that connect tissue appearance with biological context.
Following anesthesia, a cannula delivers buffered saline through the left ventricle to clear circulating blood. The fluid is then replaced with fixative, which travels through cerebral vessels, while drainage occurs from the right side of the heart. This sequence links preparation, vascular clearing, fixation, and fluid outflow in one coordinated procedure.
Brain tissue preserved this way can support histology, immunohistochemistry, and microscopy. Histology enables examination of tissue structure, immunohistochemistry supports analysis of molecular features, and microscopy provides a way to inspect anatomy and cellular organization. Together, these downstream approaches make the preparation useful for examining normal neural architecture and disease-related changes.
By preserving brain anatomy, cellular organization, and molecular features, the technique helps researchers examine disease-related changes in neural tissue with the specimen’s structure retained. This matters when microscopy or other tissue analyses are used to study those changes. Its contribution is therefore methodological: it supports accurate interpretation of neural pathology.