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Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation
 

Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation

Article DOI: 10.3791/62215-v
February 2nd, 2021

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Summary February 2nd, 2021

Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.

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