Mouse Head Alignment
Mouse Head Alignment - Related Videos
04:13
Modeling Repetitive Concussive Head Injuries in a Mouse Model
0 Views •
2025
Source: Yang, Z., et.al. A Repetitive Concussive Head Injury Model in Mice. J. Vis. Exp. (2016).This video demonstrates the induction of repetitive concussive head injuries in a mouse using an electromagnetic impact device. The procedure involves positioning the impact tip between the interfrontal and lambdoid sutures on the skull of an anesthetized mouse and using a probe tip to precisely set the impact depth. The impact is delivered at a controlled velocity and depth to simulate mild...
02:34
Mouse Headpost Implantation: A Method for Head Fixation in Rodents
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2023
This video shows a headpost implantation surgery in murine model. The headpost is used not only to head-fix and therefore restrain the mouse during experimentation, but also to securely attach a head device in freely moving animals.
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
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Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
01:17
Design Example: Alignment of a Road Line Using GIS
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2025
The alignment of a road line using Geographic Information Systems (GIS) is a critical process in civil engineering, combining advanced technology with practical decision-making. This methodology begins with the collection of geospatial data, including information on land cover, geomorphology, drainage patterns, slope, and contour details. Such data is typically acquired through satellite imagery and GIS tools, offering a comprehensive understanding of the terrain.Once the data is gathered, it...
03:01
Generating a Closed-Head Mild Traumatic Brain Injury in a Murine Model
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2025
Source: Logsdon, A. F., et al. Low-intensity Blast Wave Model for Preclinical Assessment of Closed-head Mild Traumatic Brain Injury in Rodents. J. Vis. Exp. (2020)This video demonstrates the induction of closed-head mild traumatic brain injury in a mouse model using a pressurized gas shock tube setup. The generated pressure wave ruptures the polyester membrane, delivering a shockwave to the mouse’s head that results in rapid brain movement within the skull, causing a mild traumatic brain injury.
FAQs
Bregma and lambda provide recognizable skull landmarks for establishing a consistent anatomical reference frame. Researchers use their positions to level the head and standardize anterior-posterior, medial-lateral, and dorsal-ventral coordinates. This reduces positional variation between animals, helping experimental targets remain comparable when investigators perform injections, place electrodes, or collect optical recordings.
Alignment determines how reliably coordinates correspond to the intended brain location. If the skull is not consistently leveled and secured, the same coordinate values may not produce equivalent targeting across animals. Establishing the reference frame therefore improves reproducibility and supports more dependable comparisons in experiments involving neural manipulation, circuit mapping, or brain imaging.
The same anatomical reference frame can support several experimental approaches, including injections, electrode placement, optical recordings, and behavioral imaging. Consistency is important because each technique depends on relating an intervention or measurement to a brain region. Standardized alignment reduces between-animal variation, making resulting observations easier to compare and quantitative analyses more reliable.
A typical workflow uses a stereotaxic or head-fixation apparatus to secure the skull, identifies bregma and lambda, and adjusts the head until the landmarks support a level, consistent orientation. Researchers then use the resulting reference frame to define anterior-posterior, medial-lateral, and dorsal-ventral coordinates for the planned experiment.
The procedure requires an apparatus that can secure the mouse skull and provide a stable reference for positioning. Anatomical landmarks, especially bregma and lambda, guide leveling and coordinate establishment. Together, the fixation system and landmark-based orientation create the framework needed to position experimental interventions or measurements consistently within the brain.
It is particularly useful when experiments require consistent targeting or comparison across animals. Applications described for this approach include circuit mapping, neural manipulation, injections, electrode placement, optical recordings, behavioral imaging, and quantitative analysis. By reducing positional variation, alignment strengthens interpretation of whether observed differences reflect biology rather than inconsistent experimental placement.