Neuronal Cell Recording
Neuronal Cell Recording - Related Videos
03:05
Photostimulation and Whole-Cell Patch Clamp Recordings of Neurons in Mouse Hippocampal Slices
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2025
The video demonstrates a protocol to study synaptic interactions and neuronal responses in a transfected mouse hippocampal brain slice. It involves illuminating long-range sensory neurons to trigger action potentials and recording the resulting excitatory post-synaptic potentials in the recorded neuron using the whole-cell patch-clamp technique.
05:45
Recording Electrophysiological Activity of the Neuronal Network in a Neuron-Astrocyte Co-culture
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2025
This video demonstrates establishing a neuron-astrocyte co-culture for recording neuronal network activity. Upon establishing the co-culture of motor neurons and astrocytes on a multi-electrode array (MEA) plate, the synchronous rhythmic electrical activity of the neuronal network was recorded extracellularly using the MEA.
03:34
Establishing a Whole-Cell Patch Clamp for Electrophysiological Recording from Vomeronasal Sensory Neurons
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2025
The protocol video describes establishing a whole-cell patch clamp for electrophysiological recording from the sensory neurons of the vomeronasal organ (VNO) in mice. A coronal VNO slice with fluorescently labeled sensory neurons is placed in a recording setup. A labeled neuron is patched with a recording pipette in a whole-cell configuration for electrophysiological recording in response to chemical stimuli.
02:50
Perforated Patch-Clamp Recordings of Inner Ear Sensory Neuron Cell Bodies
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2025
This video demonstrates the patch-clamp technique for measuring ionic currents in inner ear sensory neuron cell bodies. It details the preparation and use of a polished patch-clamp pipette filled with an internal solution containing amphotericin B, which perforates the membrane to allow ion movement while preserving intracellular conditions. Voltage pulses are applied to trigger and record ion channel activity, providing insights into their properties and behavior.
03:45
Whole-Cell Patch Clamp Recording in a Substantia Gelatinosa Neuron of a Spinal Cord Slice
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2025
This video demonstrates the procedure for performing electrophysiological recordings in substantia gelatinosa (SG) neurons of spinal cord slices, using whole-cell patch-clamp techniques to analyze neuron firing patterns. This method allows the study of neural responses to stimuli in SG neurons of the spinal cord.
FAQs
The electrical variable detected depends on the recording approach. A microelectrode or patch-clamp pipette can monitor membrane potential, the voltage across the neuronal membrane, or membrane current, the movement of electrical charge associated with cellular activity. Choosing between these measurements helps investigators examine different aspects of ion-channel behavior, synaptic input, and neuronal signaling.
Controlled current injection tests how readily a neuron responds to an imposed electrical change. By introducing current and observing the resulting electrical activity, investigators can evaluate excitability, including whether the cell remains at rest or generates action potentials. This approach separates intrinsic responses from activity produced by synaptic input or external sensory and pharmacological stimulation.
Recordings can distinguish a neuron at rest from one receiving synaptic input or producing action potentials. Comparing these states shows how incoming signals alter membrane electrical activity and how that activity develops into firing patterns. Such measurements connect synaptic transmission with the timing and form of neuronal output, providing cellular evidence for information processing.
Investigators compare the recorded electrical response before and after sensory or pharmacological stimulation. Changes in membrane potential, current, or firing activity indicate how the neuron responds under the tested condition. This comparison can reveal effects on ion-channel function or synaptic signaling and helps evaluate how external inputs or drugs modify neuronal communication.
The central measurement tools are a microelectrode and a patch-clamp pipette. The investigator selects the approach according to whether membrane potential or current is the main outcome, then records the neuron while it rests, receives synaptic input, or generates action potentials. Controlled current injection can be added when the goal is to test excitability.
This approach is useful when a study needs cellular evidence of abnormal neuronal signaling. Recordings can show how disease-related changes or pharmacological treatments affect ion-channel function, synaptic transmission, membrane activity, or firing patterns. Those findings help connect altered cellular physiology with broader neural dysfunction and can support evaluation of potential therapeutic effects.
Measurements from individual neurons provide a cellular level of analysis that can be related to neural-circuit function and behavior. Firing patterns, synaptic responses, and stimulation effects help researchers interpret how local neuronal activity contributes to information processing. In neuroscience, this connection supports studies of circuit mechanisms and disorders involving abnormal signaling, while informing therapy-focused research.