Stab Wound Technique
Stab Wound Technique - Related Videos
09:16
Stab Wound Injury of the Zebrafish Adult Telencephalon: A Method to Investigate Vertebrate Brain Neurogenesis and Regeneration
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Cited by 60 •
2014
To shed light on the cellular and molecular mechanisms of zebrafish adult neurogenesis and regeneration, we developed a protocol for invasive surgery causing mechanical injuries in the zebrafish adult telencephalon and subsequent monitoring of changes in the stabbed hemisphere by immunohistochemistry or in situ hybridization.
12:00
A Simplified Technique for Producing an Ischemic Wound Model
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Cited by 22 •
2012
We have developed a minimally invasive technique to create a rabbit ischemic ear wound model by dividing the central artery and nerve and the cranial neurovascular bundle. A subcutaneous tunnel then cuts all subcutaneous tissues. This procedure causes minimal skin disruption and can be safely used in diabetic animals.
04:03
Stab-Wound Mouse Model for Studying Hemorrhage and Inflammation in Traumatic Brain Injury
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Cited by 1 •
2025
Simplified traumatic brain injury (TBI) models have facilitated the development of therapeutic approaches. This protocol outlines the creation of a stab-wound mouse cortex using needles, enabling the analysis of hemorrhage and inflammation. The stab-wound TBI mouse model offers the advantage of being performed without requiring specialized equipment.
07:46
Triggering Reactive Gliosis In Vivo by a Forebrain Stab Injury
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Cited by 8 •
2015
This article describes a detailed protocol to produce a forebrain stab injury in adult mice. The stab injury induces severe reactive gliosis and glial scar formation which can be subsequently examined by standard immunohistochemistry methods.
08:35
Demonstration of the Rat Ischemic Skin Wound Model
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Cited by 32 •
2015
The rat, due to its size, availability, and rather docile behavior, has been utilized as a research model for many years. The goal of this protocol is to utilize the rat as an ischemic skin wound healing model to provide valuable insight into the pathophysiology of chronic wounds.
FAQs
The lesion disrupts cells and tissue architecture, creating a localized signal of neural damage. This injury activates inflammation, glial responses, and repair processes within the affected tissue. Examining these coordinated reactions allows researchers to study how neural and non-neural cells respond after damage, rather than analyzing neuronal loss in isolation.
Blood-brain barrier disruption provides a way to examine how injury changes the boundary between neural tissue and the circulation. Because the lesion is localized and reproducible, investigators can relate barrier changes to nearby cellular and tissue responses. This supports analysis of damage mechanisms and repair processes relevant to neurological injury.
Standardizing the injury location and extent makes results more comparable across experimental conditions. Researchers can evaluate whether differences in inflammation, glial activity, regeneration, or degeneration reflect the variable being tested rather than inconsistent tissue damage. This reproducibility is especially valuable when comparing responses among neural regions or experimental groups.
The model supports examination of interactions between neurons and non-neuronal cells after tissue damage. Such interactions can be considered alongside inflammation, glial responses, and repair activity to clarify how neural tissue reacts as a coordinated system. This perspective helps connect cellular behavior with broader outcomes such as neurodegeneration and neuronal regeneration.
A sterile blade or needle is inserted into neural tissue to a defined depth, producing a localized lesion with a controlled extent. The selected instrument, insertion depth, and injury location are therefore central experimental features. Maintaining these parameters allows investigators to generate comparable injuries and assess responses under different experimental conditions.
Researchers may use the model when they need to investigate neurodegeneration, blood-brain barrier disruption, neuronal regeneration, or repair after localized damage. Its reproducible lesion also supports comparisons between experimental conditions. These applications make it useful for studying how neural tissue responds to injury and for evaluating mechanisms that may inform neurological therapies.
The technique provides a controlled context for examining inflammation, glial responses, barrier disruption, degeneration, regeneration, and repair following neural injury. Although it produces a localized experimental lesion, the resulting mechanisms may help inform research on traumatic brain injury and other neurological disorders. Its value lies in connecting defined tissue damage with measurable biological responses.