Ex Vivo Preparation
Ex Vivo Preparation - Related Videos
12:00
Spatiotemporal Mapping of Motility in Ex Vivo Preparations of the Intestines
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Cited by 26 •
2016
Recently available video recording and spatiotemporal mapping (STmap) techniques make it possible to visualize and quantify both propagating and mixing patterns of intestinal motility. The goal of this protocol is to explain the generation and analysis of STmaps using the GastroIntestinal Motility Monitoring (GIMM) system.
07:00
The ex vivo Isolated Skeletal Microvessel Preparation for Investigation of Vascular Reactivity
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Cited by 20 •
2012
An ex vivo preparation is described for isolation of the largest gracilis muscle resistance arterioles for interrogation of both vascular responses to vasoactive stimuli and the assessment of basic structural properties via passive wall mechanics.
08:18
Preparation of Naringenin Solution for In Vivo Application
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2021
Here, the protocol presents the preparation of naringenin solution for in vivo intraperitoneal administration. Naringenin is fully dissolved in a mixture of dimethylsulfoxide, Tween 80, and saline. The antidiabetic osteoporotic effects of naringenin were assessed by blood glucose testing, tartrate-resistant acid phosphatase staining, and enzyme-linked immunosorbent assay.
05:55
Whole Lung Agarose Inflation: A Method of Preparing Lungs for Ex vivo Live Imaging
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2023
This video describes a method of preparing lungs for ex vivo live imaging to study interactions between cancer cells and stromal cells, using agarose for inflation. In the sample protocol, agarose inflation procedure is described before observing live cellular interactions.
08:07
Preparation of Herbal Medicine: Er-Xian Decoction and Er-Xian-containing Serum for In Vivo and In Vitro Experiments
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Cited by 6 •
2017
Here, we present the preparation of an er-xian decoction (EXD) in four steps—soaking, decoction, filtration, and concentration—and demonstrate the administration of a prepared EXD-containing serum to rats. These methods are applicable to the in vivo and in vitro study of herbal decoctions such as traditional Chinese medicines.
FAQs
Maintaining an oxygenated physiological solution helps preserve neural tissue after removal by supplying a controlled environment in which cellular structure and function can remain usable for study. Temperature, nutrients, and related conditions must also be regulated because the preparation no longer benefits from the organism’s integrated internal environment. This control supports measurements of neuronal activity and synaptic mechanisms.
The outcome depends on preserving several conditions simultaneously, including oxygenation, temperature, nutrient availability, and the composition of the physiological solution. These variables help maintain the tissue in a state suitable for experimental observation. Careful regulation is especially important when researchers examine cellular function, synaptic transmission, or neural circuits, because altered conditions could affect the behavior being measured.
Isolation reduces the complexity created by whole-animal influences and allows researchers to study defined neural tissues under controlled conditions. This approach makes it easier to connect an observed response with a cellular, synaptic, or circuit-level mechanism. The resulting measurements can therefore focus on local neural processes rather than the combined effects of the entire organism.
Researchers begin by rapidly dissecting the selected brain or neural tissue, then placing it in an oxygenated physiological solution. They maintain suitable temperature, nutrient, and other environmental conditions while preparing the tissue for measurement. This sequence is designed to retain cellular structure and function long enough for electrophysiology, microscopy, pharmacological testing, or circuit analysis.
The core requirements are the excised neural tissue, an oxygenated physiological solution, and controlled environmental conditions. Temperature and nutrient availability must be regulated, along with other properties of the surrounding solution that help preserve tissue function. Together, these conditions create a stable experimental setting in which researchers can collect measurements without relying on the full internal environment of the organism.
These preparations support several complementary approaches, including electrophysiology, microscopy, pharmacological testing, and analysis of synaptic transmission. Electrophysiology examines neural electrical activity, whereas microscopy reveals tissue or cellular features. Pharmacological experiments test responses to applied compounds, and synaptic or circuit studies investigate communication between neurons and the organization of neural pathways.
An ex vivo preparation is useful when researchers need precise access to defined neural tissue while reducing whole-animal complexity. It can support focused studies of synaptic transmission and neural circuits under regulated conditions, allowing investigators to relate experimental manipulations to measurable cellular or network responses. The approach is therefore valuable for connecting controlled observations with specific neuroscience mechanisms.