Vascular Isolation
Vascular Isolation - Related Videos
08:24
Isolation of Murine Coronary Vascular Smooth Muscle Cells
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Cited by 11 •
2016
The purpose of this protocol is to demonstrate the isolation and culture techniques of murine primary vascular smooth muscle cells (VSMCs) from the coronary circulation. Once VSMCs have been isolated, they can be used for many standard culture techniques.
07:22
Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
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Cited by 151 •
2013
Primary white preadipocytes isolated from white adipose tissues in mice can be differentiated into beige/brite cells. Presented here is a reliable cellular model system to study the molecular regulation of "browning" of white fat.
07:00
The ex vivo Isolated Skeletal Microvessel Preparation for Investigation of Vascular Reactivity
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Cited by 20 •
2012
An ex vivo preparation is described for isolation of the largest gracilis muscle resistance arterioles for interrogation of both vascular responses to vasoactive stimuli and the assessment of basic structural properties via passive wall mechanics.
10:28
Evaluation of Vascular Control Mechanisms Utilizing Video Microscopy of Isolated Resistance Arteries of Rats
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2017
This manuscript describes in vitro video microscopy protocols for evaluating vascular function in rat cerebral resistance arteries. The manuscript also describes techniques for evaluating microvessel density with fluorescently labeled lectin and tissue perfusion using Laser Doppler Flowmetry.
07:09
Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering
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Cited by 58 •
2017
We present a protocol to isolate adipose tissue-derived microvascular fragments that represent promising vascularization units. They can be rapidly isolated, do not require in vitro processing and, thus, may be used for one-step prevascularization in different fields of tissue engineering.
FAQs
Mechanical separation releases vessels through physical dissection, whereas enzymatic separation uses digestion to help detach them from surrounding brain tissue. The choice of approach can affect how well vascular structures and cellular components remain available for study. Both methods are used to obtain cerebral vessels for examining structure, cellular composition, and function independently from intact neural tissue.
Maintaining endothelial cells, pericytes, and associated basement membrane preserves key parts of the neurovascular unit rather than reducing the preparation to vessel structure alone. This allows researchers to examine vascular signaling, blood-brain barrier properties, inflammation, and responses to injury in relation to the cellular components that support cerebral vessel function.
Isolated vessels provide a focused system for investigating how the blood-brain barrier operates and how cerebral vascular cells participate in signaling and inflammation. Because the vessels are separated from surrounding tissue, researchers can examine vascular responses more directly. This helps connect changes in vessel biology with injury, disease processes, and altered neurovascular function.
A typical workflow begins by dissecting the relevant brain tissue, followed by mechanical or enzymatic separation to release the vessels. The isolated material is then maintained with attention to retaining endothelial cells, pericytes, and basement membrane. This preparation can subsequently supply material for molecular analysis, cell culture, imaging, or pharmacological studies.
The resulting cerebral vessel preparation can be used for molecular analysis of vascular components, cell culture studies, imaging, and pharmacological testing. These approaches provide complementary information about vessel structure, cellular composition, signaling, barrier-related properties, inflammation, and responses to injury. Using several readouts can connect molecular changes with observable vascular behavior.
This approach is useful when researchers need to examine vascular contributions to stroke, neurodegeneration, or brain tumors. Studying cerebral vessels separately helps focus on how vascular dysfunction, inflammatory signaling, barrier changes, or injury responses may contribute to these conditions. The isolated material also supports pharmacological studies aimed at understanding or testing vascular responses.