הכנס גדול הסביבה הגנום ספריית הפקה

0:07

Introduction

0:43

End Repair of Sheared Genomic DNA

4:48

DNA-recovery by Gel Extraction and Ligation of Recovered DNA to the Fosmid Cloning Vector

20:32

Conclusion

2:10

Size-Selection of Genomic DNA by Pulsed-Field Gel Electrophoresis (PFGE)

10:18

Phage-packaging of Vector-insert Ligation Product and Infection of Phage-Resistant Bacteria

17:46

Colony Picking, Culturing, and Long-Term Storage

14:30

Plating and Titering

16:34

Preparation for Colony Picking