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November 07, 2012
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שיטת אבולוציה מכוונת רומן ספציפית לתחום הנדסת thermostability פותחה וכתוצאה מכך תוקפת לאנזימי bacteriolytic. לאחר סיבוב אחד בלבד של mutagenesis האקראי, אנזים bacteriolytic התפתח, PlyC 29C3, מוצג יותר מכפולה מפעילות שיורית בהשוואה לחלבון פרא מהסוג לאחר דגירת טמפרטורה גבוהה.
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Cite this Article
Heselpoth, R. D., Nelson, D. C. A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes. J. Vis. Exp. (69), e4216, doi:10.3791/4216 (2012).
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