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August 04, 2016
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We describe a targeted RNA sequencing-based method that includes preparation of indexed cDNA libraries, hybridization and capture with custom probes and data analysis to interrogate selected transcripts for gene expression, mutations, and gene fusions. Targeted RNAseq permits cost-effective, rapid evaluation of selected transcripts on a desktop sequencer.
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Cite this Article
Martin, D. P., Miya, J., Reeser, J. W., Roychowdhury, S. Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations. J. Vis. Exp. (114), e54090, doi:10.3791/54090 (2016).
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