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05:48 min
March 16, 2022
DOI:
10.3791/63405-v
הפרוטוקול הנוכחי מתאר מוטציה אחת M213L ב- Gja1 השומרת על דור Connexin43 באורך מלא אך מונעת תרגום של איזופורם קטן יותר של GJA1-20k שתורגם באופן פנימי.
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Cite this Article
Shimura, D., Hunter, J., Katsumata, M., Shaw, R. M. Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein. J. Vis. Exp. (181), e63405, doi:10.3791/63405 (2022).
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