JOVE JOURNAL

Developmental Biology

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Journal (Developmental Biology)

In Vitro Culturing Technique for Studying Cellular Dynamics in Zebrafish Scales

Juan D. Carvajal-Agudelo¹, Tamara A. Franz-Odendaal¹

¹Department of Biology,Mount Saint Vincent University

Journal (Developmental Biology)

Manipulating Mechanical Forces in the Developing Zebrafish Heart Using Magnetic Beads

Christina Vagena-Pantoula^1,2, Hajime Fukui^3,4, Julien Vermot^1,2

¹Department of Bioengineering,Imperial College London, ²The Francis Crick Institute, ³Institute of Advanced Medical Sciences,Tokushima University, ⁴Department of Cell Biology,National Cerebral and Cardiovascular Center Research Institute

Journal (Developmental Biology)

Fibro-Adipogenic Progenitor Isolation, Expansion, and Differentiation from the Spiny Mouse Model

Bruce Lin¹, Hesham Soliman^1,2, Fabio M. V. Rossi¹, Marine Theret¹

¹School of Biomedical Engineering and Department of Medical Genetics,University of British Columbia, ²Aspect Biosystems

Journal (Developmental Biology)

Preparation of Frozen Non-Human Primate Fetal Islets for Combined Single Nuclei RNA-Sequencing and ATAC-Sequencing, and Bulk Metabolomics

Darian T. Carroll¹, Sarah R. Lindsley², Melissa Kirigiti², Alex Buko³, Angela Jones⁴, Paul Kievit², Maureen Gannon^1,5,6,7

¹Department of Molecular Physiology and Biophysics,Vanderbilt University, ²Division of Metabolic Health and Disease,Oregon National Primate Research Center, ³Human Metabolome Technologies (HMT) America, ⁴Vanderbilt Technologies for Advanced Genomics,Vanderbilt University Medical Center, ⁵Department of Medicine,Vanderbilt University Medical Center, ⁶Department of Veterans Affairs Tennessee Valley, ⁷Department of Cell and Developmental Biology,Vanderbilt University

Journal (Developmental Biology)

Z-Scores for Assessing Ovarian Reserve in Young Patients Undergoing Fertility Preservation

Jasmin Hassan^1,2, Pauliina Damdimopoulou^1,2, Atte Lahtinen^3,4, Kirsi Jahnukainen^5,6, Katri Knuus⁷, Ganesh Acharya¹, Richelle D. Björvang^1,8,9

¹Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology,Karolinska Institutet, ²Department of Gynecology and Reproductive Medicine,Karolinska University Hospital, ³Applied Tumor Genomics Research Program, Faculty of Medicine,University of Helsinki, ⁴Department of Medical and Clinical Genetics, Medicum, Faculty of Medicine,University of Helsinki, ⁵Children's Hospital, Pediatric Research Centre,University of Helsinki and Helsinki University Hospital, ⁶NORDFERTIL Research Lab Stockholm, Department of Women's, and Children's Health,Karolinska Institutet, and Karolinska University Hospital, ⁷Department of Obstetrics and Gynecology, Helsinki University Hospital,University of Helsinki, ⁸Department of Cell and Molecular Biology,Uppsala University, ⁹Department of Women's and Children's Health,Uppsala University

Journal (Developmental Biology)

Quantifying Drosophila melanogaster Eye Phenotypes: A Computational Approach Integrating ilastik and Flynotyper

Qasim Mujteba*¹, Madeleine R. Chalmers*¹, JiHye Kim¹, Minwoo Baek¹, Nam Chul Kim¹

¹Department of Pharmacy Practice and Pharmaceutical Sciences, College of Pharmacy,University of Minnesota

Journal (Developmental Biology)

Formation of Human Thymus Organoids in Three-Dimensional Fibrin Hydrogels

Manon d'Arco*¹, Nathan Provin*¹, Pierre Maminirina², Olivier Baron², Carole Guillonneau¹, Laurent David^1,3, Matthieu Giraud¹

¹Nantes Université, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, ²CHU Nantes, Nantes Université, Department of Pediatric and Congenital Cardiac Surgery, ³Nantes Université, CHU Nantes, INSERM, CNRS, BioCore, SFR Bonamy

Journal (Developmental Biology)

Two-Photon Laser-Mediated Ablation of Osteoblasts in Developing Zebrafish Larvae

Alejandra Cristina López-Delgado^1,2, Franziska Knopf^1,2

¹CRTD - Center for Regenerative Therapies TU Dresden, ²Center for Healthy Aging, Faculty of Medicine,TU Dresden

Journal (Developmental Biology)

Human Ovarian Surface Epithelium Organoids as a Platform to Study Tissue Regeneration

Julieta S. Del Valle^1,2, Azra Husetic*¹, Dina Diek*¹, Laurens F. Rutgers*¹, Joyce D. Asseler^3,4,5, Jeroen Metzemaekers⁶, Norah M. van Mello^3,4,5, Susana M. Chuva de Sousa Lopes^1,2,7

¹Department of Anatomy and Embryology,Leiden University Medical Center, ²The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW),Leiden University Medical Center, ³Department of Obstetrics and Gynaecology,Amsterdam University Medical Center, ⁴Centre of Expertise on Gender Dysphoria,Amsterdam UMC, ⁵Amsterdam Reproduction and Development Research Institute, ⁶Department of Gynaecology,Leiden University Medical Center, ⁷Ghent-Fertility and Stem Cell Team (G-FAST), Department of Reproductive Medicine,Ghent University Hospital

Journal (Developmental Biology)

Rapid Isolation of Stage I Oocytes in Zebrafish Devoid of Granulosa Cells

Qianwen Zheng¹, Xiaolong Xie¹, Yao Li¹, Chengbo Ai¹, Siyu Pu¹, Jing Chen¹

¹Department of Pediatric Surgery and Laboratory of Pediatric Surgery, West China Hospital / West China School of Medicine,Sichuan University

Journal (Developmental Biology)

Light Sheet Microscopy Imaging and Mounting Strategies for Early Zebrafish Embryos

Swetha Nagarajan*¹, Siddhartha Bardhan*¹, Sundar Ram Naganathan¹

¹Department of Biological Sciences,Tata Institute of Fundamental Research

Journal (Developmental Biology)

High-resolution Cell Transplantation in Embryonic and Larval Zebrafish

Lindsey S. Qian¹, Rabab Ibrahim^1,2, Adam J. Isabella^1,2

¹Department of Genetics, Cell Biology, and Development,University of Minnesota, ²Graduate Program in Molecular, Cellular, Developmental Biology and Genetics,University of Minnesota

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