29.7
Panoramica delle giunzioni cellula-cellula
La complessa disposizione tridimensionale delle cellule in qualsiasi organismo multicellulare è definita e mantenuta dalle interazioni delle cellule t…
Le giunzioni cellulari sono grandi complessi multiproteici che possono collegare le cellule alla matrice extracellulare formando giunzioni cellula-matrice, o fungere da punti di contatto tra le cellule vicine nei tessuti per formare giunzioni cellula-cellula.
Le celle hanno tipicamente tre tipi principali di giunzioni cellula-cellula: giunzioni strette, di ancoraggio e a fessura, ciascuna con una struttura e una funzione distinte.
Consideriamo l'epitelio colonnare. Le giunzioni strette vicino agli apici di queste cellule tengono insieme le membrane plasmatiche adiacenti e fungono da barriera al flusso di molecole attraverso gli spazi intercellulari.
Al di sotto di questa porzione apicale ci sono due tipi di giunzioni di ancoraggio: le giunzioni aderenti e i desmosomi.
Insieme, queste giunzioni di ancoraggio formano una rete citoscheletrica interconnessa tra le cellule che aiuta a resistere alle forze meccaniche.
Il terzo tipo, le giunzioni gap, sono disperse in tutta la membrana plasmatica, in particolare verso l'estremità basale. Queste giunzioni fungono da canali di comunicazione tra cellule adiacenti, consentendo il passaggio di molecole e ioni.
View the full transcript and gain access to JoVE Core videos
Q1: What are the three main types of cell-cell junctions?
Cell-cell junctions are classified into three main types based on function: tight junctions, anchoring junctions, and gap junctions. Tight junctions prevent molecule movement through intercellular spaces. Anchoring junctions hold cells together and provide mechanical strength through cytoskeletal connections. Gap junctions facilitate communication between adjacent cells by allowing molecules and ions to pass through protein channels.
Q2: How do tight junctions function as barriers in epithelial tissues?
Tight junctions hold adjacent plasma membranes in close proximity near the apices of epithelial cells, preventing the flow of molecules through intercellular spaces. They consist of transmembrane proteins that directly interact with proteins on adjacent membranes. Tight junctions can also be selectively permeable, allowing charge-specific ions to move through while blocking others.
Q3: What is the role of anchoring junctions in maintaining tissue structure?
Anchoring junctions hold cells together and provide mechanical strength by forming contact points where the cytoskeleton of one cell anchors to neighboring cells via transmembrane adhesion molecules. There are two types: adherens junctions linking the actin cytoskeleton and desmosomes linking intermediate filaments. Together, they form an extensive cytoskeletal network that resists mechanical forces and maintains tissue integrity.
Q4: How do gap junctions enable cell-to-cell communication?
Gap junctions are protein channels that link the cytoplasms of adjacent cells, allowing the exchange of molecules, ions, and electrical impulses. The constituent transmembrane proteins form channels whose opening and closing regulates molecular movement. For example, cardiac muscle cells use gap junctions to pass signals for rhythmic heart contractions from cell to cell.
Q5: What is the difference between gap junctions and plasmodesmata?
Gap junctions in animal cells allow direct exchange of molecules and ions between adjacent cell cytoplasms. Plant cells cannot use gap junctions due to their cell walls; instead, they form plasmodesmata—membrane-lined pores connecting cells through cell walls. In plasmodesmata, the cytoplasm of one cell is contiguous with that of another, allowing free movement of solutes and ions.
Q6: How are anchoring junctions organized in columnar epithelial cells?
In columnar epithelium, anchoring junctions are positioned below the apical tight junctions and consist of two types: adherens junctions and desmosomes. These anchoring junctions form an interconnected cytoskeletal network across cells that helps resist mechanical forces. Together, they maintain the structural integrity and function of the epithelial tissue.
Q7: What role do multi-protein complexes play in cell junctions?
Cell junctions are large, multi-protein complexes where proteins on one cell interact with proteins on an adjacent cell. These complexes link cells to each other or to the extracellular matrix, forming specialized contact points. The specific arrangement and composition of these multi-protein complexes determine the junction type and its function in maintaining tissue organization.