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Elettroforesi su gel bidimensionale
L'elettroforesi su gel bidimensionale è un metodo di separazione delle proteine ad alta risoluzione introdotto per la prima volta da O' Farrell e Klos…
L'elettroforesi su gel bidimensionale combina due dimensioni per la separazione delle proteine: la prima in base alla carica e la seconda in base alla massa.
La prima dimensione utilizza il metodo della focalizzazione isoelettrica o IEF. Qui, il campione proteico viene caricato su un gradiente di pH immobilizzato o su una striscia IPG.
Applicando la corrente elettrica, le proteine si muovono attraverso il gradiente di pH sulla striscia, immobilizzandosi nel loro punto isoelettrico, il pH, dove le proteine non trasportano alcuna carica netta.
Successivamente, la striscia IPG viene trattata con SDS e caricata su un gel di poliacrilammide per la separazione mediante la seconda dimensione utilizzando SDS-PAGE.
In una direzione perpendicolare all'IEF, le proteine si separano elettroforeticamente in base alla massa.
Le proteine separate vengono quindi visualizzate dopo la colorazione.
L'elettroforesi su gel bidimensionale è una tecnica ad alta risoluzione in grado di identificare proteine simili che differiscono anche per un residuo di amminoacido carico.
Inoltre, è in grado di rilevare le modifiche proteiche all'interno di una cellula o di un organello durante diverse condizioni e fasi di sviluppo.
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Q1: What are the two dimensions used in two-dimensional gel electrophoresis?
Two-dimensional gel electrophoresis separates proteins by charge in the first dimension and by mass in the second dimension. The first dimension uses isoelectric focusing (IEF) on an immobilized pH gradient strip, where proteins migrate until reaching their isoelectric point—the pH where they carry no net charge. The second dimension uses SDS-PAGE, separating proteins electrophoretically by mass perpendicular to the first dimension.
Q2: How does isoelectric focusing separate proteins on an IPG strip?
Isoelectric focusing uses an immobilized pH gradient (IPG) strip to separate proteins based on their charge. When electric current is applied, amphoteric protein molecules migrate across the pH gradient until each protein reaches its isoelectric point, where it carries no net charge and becomes immobilized. This charge-based separation is the first dimension of two-dimensional gel electrophoresis.
Q3: What is the purpose of treating the IPG strip with SDS before the second dimension?
After isoelectric focusing, the IPG strip is treated with sodium dodecyl sulfate (SDS) buffer to prepare proteins for mass-based separation. SDS coats the uncharged proteins with a negative charge, enabling them to migrate uniformly through the polyacrylamide gel. Glycerol in the buffer reduces electroendosmosis, facilitating protein transfer from the first to the second dimension.
Q4: Why is two-dimensional gel electrophoresis considered a high-resolution technique?
Two-dimensional gel electrophoresis achieves high resolution by separating proteins along two independent parameters: charge and mass. This dual-dimension approach can identify similar proteins differing by even one charged amino acid residue. The technique also detects protein modifications within cells or organelles during different conditions and developmental stages, providing detailed protein analysis.
Q5: What buffer components are used to prepare biological samples for isoelectric focusing?
Biological samples are prepared using a buffer containing urea, dithiothreitol, detergents, and ampholytes. Urea and detergent solubilize and denature proteins, while dithiothreitol, a reducing agent, cleaves disulfide bonds. Ampholytes solubilize proteins and maintain pH, while the buffer rehydrates the IPG strip to help it absorb proteins before charge-based separation.
Q6: What are the limitations of two-dimensional gel electrophoresis for protein analysis?
Two-dimensional gel electrophoresis is unsuitable for distinguishing highly hydrophobic proteins, proteins with high molecular mass, or those present in very low quantities per cell. Additionally, narrower pH gradient IPG strips (such as pH 4-7) detect proteins with higher accuracy than broader-range strips (pH 3-10), requiring careful selection based on sample composition.
Q7: How are separated proteins visualized after two-dimensional gel electrophoresis?
Separated proteins are visualized through multiple methods depending on sample preparation. General staining techniques reveal protein bands on the gel. Radiolabeled samples produce autoradiographic images, while fluorescently labeled samples generate fluorescent images. These visualization approaches enable identification and analysis of the separated protein spots on the gel.