Generating a Mouse Model of Traumatic Brain Injury Using an Impactor Device

Abstract

Source: Schwulst, S. J. et al. Murine Model of Controlled Cortical Impact for the Induction of Traumatic Brain Injury. J. Vis. Exp. (2019)

This video demonstrates the procedure for inducing traumatic brain injury (TBI) in a mouse model. An anesthetized mouse with an exposed skull is taken to create an opening in the skull, and then a controlled cortical injury is induced using an impact device. The injury damages the brain tissue, causing impaired cognitive function and motor coordination.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Induce anesthesia

1. Anesthetize the mouse with ketamine (125 mg/kg) and xylazine (10 mg/kg) injected intraperitoneally.

2. Vital signs monitoring every 15 min

1. Monitor temperature, respiratory rate, and skin color. The mouse should feel warm to the touch. The skin should appear pink and well perfused. The respiratory rate should range 50–70 breaths per minute.

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Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
AnaSed Injection Xylazine Sterile Solution	LLOYD, Inc.	5939911020
Buprenorphine SR Lab 0.5mg/mL	Zoopharm-Wildlife Pharmaceuticals USA	BSRLAB0.5-182012
High Speed Rotary Micromotor KiT0	Foredom Electric Company	K.1070
Imapact one for Stereotaxix CCI	Leica Biosystems Nussloch GmbH	39463920
Ketathesia Ketamine HCl Injection USP	Henry Schein, Inc	56344
Mouse Specific Stereotaxic Base	Leica Biosystems Nussloch GmbH	39462980
Trephines for Micro Drill	Fine Science Tools, Inc	18004-50