Hairpin Vortices

Hairpin vortices are coherent, hairpin-shaped structures in fluid flow that help organize turbulence and transport momentum near a surface. They form when elongated streamwise vortices lift low-momentum fluid away from a wall, creating an upward-moving ejection beneath a curved vortex head and associated downward sweeps of faster fluid. In bioengineering, understanding these structures supports analysis of flow through blood vessels, airways, and engineered channels, where turbulence can influence wall shear stress, mixing, particle transport, and device performance. Their study also informs computational models and experimental designs for controlling fluid behavior in biomedical systems.

Hairpin Vortices - Related Videos

Research

JoVE Journal - Engineering
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Preparation of Free-Surface Hyperbolic Water Vortices

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Cited by 6 •

2023

This paper describes how three different water vortex regimes in a hyperbolic Schauberger funnel can be created, their most important characteristics, and how associated parameters such as the oxygen transfer rates can be calculated.

Research

JoVE EoE - Genome Editing Techniques

Short Hairpin RNA-Mediated Gene Knockdown in iHSPCs In Vitro: A Lentivirus-Based shRNA Expression System Delivery into iHSPCs for Knockdown of Specific Gene Expression

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2025

In this video, we demonstrate a method to perform transduction of shRNA lentiviral vectors to obtain stable knockdown cell lines in immortalized hematopoietic stem and progenitor cells (iHSPCs).

Assessing Intracardiac Vortices with High Frame-Rate Echocardiography-Derived Blood Speckle Imaging in Newborns

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Cited by 1 •

2023

The present protocol uses echocardiography-derived blood speckle imaging technology to visualize intracardiac hemodynamics in newborns. The clinical utility of this technology is explored, the rotational body of fluid within the left ventricle (known as a vortex) is accessed, and its significance in understanding diastology is determined.

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

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Cited by 6 •

2020

We provide a protocol to stably knock down genes encoding extracellular matrix (ECM) proteins in C2C12 myoblasts using small-hairpin (sh) RNA. Targeting ADAMTSL2 as an example, we describe the methods for the validation of the knockdown efficiency on the mRNA, protein, and cellular level during C2C12 myoblast to myotube differentiation.

Efficient Gene Knockdown in the Liver via Intrasplenic Injection of Adeno-Associated Virus Serotype 8 (AAV8)-Delivered Small Hairpin RNA

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2024

This protocol describes how intrasplenic injection of AAV8-delivered small hairpin RNA achieves the same gene knockdown efficiency in the liver as portal vein injection, representing a simpler procedure with much lower perioperative and postoperative mortality and complications.

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