Scaffold Strand
Scaffold Strand - Related Videos
04:33
Decellularized Porcine Esophageal Scaffold Preparation: A Technique to Generate Acellular Scaffold from a Pig Esophagus
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2023
In this video, we demonstrate the preparation of a decellularized pig esophagus scaffold, with its biochemical architecture and composition preserved, for tissue engineering applications.
02:04
Fixing Double-strand Breaks
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2020
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
09:32
Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
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Cited by 5 •
2015
Here, we present a protocol to fabricate electrospun nanofiber scaffolds with gradated organization of fibers and explore their applications in regulating cell morphology/orientation. Gradients with regard to physical and chemical properties of the nanofiber scaffolds offer a wide variety of applications in the biomedical field.
08:35
Elastomeric PGS Scaffolds in Arterial Tissue Engineering
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Cited by 21 •
2011
Elastomeric PGS scaffolds with vascular smooth muscle cells cultured in a pulsatile flow bioreactor may lead to promising small-diameter arterial constructs with native ECM production in a relatively short culture period.
14:49
Self-reporting Scaffolds for 3-Dimensional Cell Culture
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Cited by 7 •
2013
Biocompatible pH responsive sol-gel nanosensors can be incorporated into poly(lactic-co-glycolic acid) (PLGA) electrospun scaffolds. The produced self-reporting scaffolds can be used for in situ monitoring of microenvironmental conditions whilst culturing cells upon the scaffold. This is beneficial as the 3D cellular construct can be monitored in real-time without disturbing the experiment.
FAQs
Scaffold strand diameter and surface properties can change how cells attach, migrate, and interact with a construct. Diameter contributes to the strand’s structural scale, while surface properties influence the interface presented to cells. Because these features also affect scaffold architecture, researchers tune them together to support suitable mechanical behavior, cellular organization, and tissue development.
Arrangement determines how individual strands contribute to the scaffold’s three-dimensional architecture. Bundling, weaving, and alignment can be adjusted to influence mechanical strength, porosity, and fluid transport, while also organizing the environment encountered by cells. Selecting among these configurations helps researchers reproduce structural features of native tissues and guide tissue formation within the construct.
Combining scaffold strands with hydrogels allows a construct to incorporate fibrous architecture alongside another biomaterial phase. This combination can help reproduce structural features of native tissues while providing additional opportunities to tune material properties and cell interactions. Researchers can therefore use mixed-material designs to support cell growth, tissue formation, and biomimetic scaffold development.
A design process can begin by selecting the strand composition and diameter, then adjusting surface properties and the overall arrangement. Researchers may bundle, weave, or align the strands, or combine them with hydrogels and other materials. These choices are coordinated to balance mechanical strength, porosity, fluid transport, cell behavior, and tissue-development goals.
Scaffold strands are useful when researchers need organized biomaterials for tissue-engineering constructs or other three-dimensional bioengineering designs. They support work on engineered replacements, regenerative therapies, and biomimetic devices. Their tunable architecture makes them relevant when a study must connect material structure with cell growth, guided tissue formation, or tissue-like organization.
Evaluation can examine mechanical strength, porosity, and fluid transport, along with cell attachment, migration, and interaction with the scaffold. Researchers can also assess whether the design supports cell growth and guides tissue formation. Together, these outcomes show how strand composition, dimensions, surface properties, and arrangement affect the construct’s intended bioengineering function.