Shear Flow Clots
Shear Flow Clots - Related Videos
06:16
Tracking Fibrinolysis of Chandler Loop-Formed Whole Blood Clots Under Shear Flow in An In-Vitro Thrombolysis Model
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2024
In-vitro thrombolysis assays have often struggled to replicate in-vivo conditions whether in the model thrombus being digested or in the environment in which thrombolysis is occurring. Herein, we explore how coupling the Chandler loop and Real-Time Fluorometric Flowing Fibrinolysis Assay (RT-FluFF) is used for high-fidelity, ex-vivo, clot lysis monitoring.
09:20
The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
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Cited by 10 •
2016
Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.
12:43
Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
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Cited by 51 •
2012
We are describing a method to subject adherent cells to laminar flow shear stress in a sterile continuous flow circuit. The cells' adhesion, morphology can be studied through the transparent chamber, samples obtained from the circuit for metabolite analysis and cells harvested after shear exposure for future experiments or culture.
08:01
The Diffusion of Passive Tracers in Laminar Shear Flow
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Cited by 4 •
2018
A protocol for the study of the diffusion of passive tracers in laminar pressure-driven flow is presented. The procedure is applicable to various capillary pipe geometries.
03:31
Inducing Blood Clot Formation in the Mouse Brain Using a Photosensitive Dye
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2025
Source: Talley Watts, L., et al. Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke. J. Vis. Exp. (2015)This video demonstrates a technique to induce blood clot formation in a mouse brain. An anesthetized mouse with a cranial window and a thinned skull undergoes photothrombosis through the activation of a photosensitive dye using high-intensity light. This process generates reactive oxygen species, which damage the vessel walls and trigger clot...
FAQs
Hydrodynamic forces affect how platelets move through the flowing fluid and encounter a damaged surface. They influence the sequence of platelet adhesion, activation, and aggregation as the clot develops. Controlling these forces allows researchers to examine how changing shear conditions alters platelet function and thrombus growth in a system designed to reflect aspects of circulation.
Fibrin contributes structural stability to the developing thrombus. Platelets can adhere, activate, and aggregate under flow, while fibrin formation helps stabilize the resulting clot. Measuring clot behavior with and without attention to this stabilizing process helps researchers distinguish early platelet responses from later changes associated with a more established thrombus.
Controlled shear rates provide a defined hydrodynamic environment for comparing clot formation between experiments. Because flow affects platelet transport, surface adhesion, activation, and aggregation, changing the shear rate can change the measured response. This control makes microfluidic and flow-chamber experiments useful for linking specific flow conditions with clot growth and platelet function.
Researchers use microfluidic platforms or flow chambers to reproduce selected shear rates while blood or blood components move across a damaged surface or engineered material. These systems support measurements of clot growth, platelet function, and material interactions under controlled conditions. The resulting observations provide laboratory data that can be compared across experiments and related to clot behavior in circulation.
These models are useful when researchers need to evaluate how blood responds to engineered surfaces or devices under moving-fluid conditions. Applications include studying vascular disease, testing blood-contacting medical devices, examining antithrombotic therapies, and assessing biomaterial safety. Their controlled flow environment helps reveal clot-related behavior that may be missed by evaluating materials without circulation-like forces.
A shear-flow experiment can provide information about clot growth, platelet function, and the interaction between developing thrombi and engineered materials. These measurements help researchers assess how controlled flow conditions influence clot formation and stability. In bioengineering, the findings can connect laboratory observations with expected clot behavior in the circulation and inform device or therapy research.