The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

The Assembly and Application of ‘Shear Rings’: A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

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Biology

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JoVE Journal Biology

The Assembly and Application of ‘Shear Rings’: A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

DOI:

10.3791/54632-v

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09:20 min

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October 31, 2016

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Luke A. White, Emily V. Stevenson, J. Winny Yun, Randa Eshaq, Norman R. Harris, David K. Mills, Alireza Minagar, Pierre-Olivier Couraud, J. Steven Alexander

¹Molecular and Cellular Physiology,Louisiana State University Health Sciences Center in Shreveport, ²Biological Sciences,Louisiana Tech University, ³Neurology,Louisiana State University Health Sciences Center in Shreveport, ⁴Institut Cochin, Inserm U1016, Cnrs Umr8104,Université Paris Descartes

Chapters

00:05Title
00:45Construction of 150-mm Diameter Shear Rings
04:57Sterilization of Shear Rings
06:31Shear Stress Studies
07:40Results: Shear Rings Effectively Create Unidirectional and Periodic Shear Stress
08:29Conclusion

Summary

Automatic Translation

Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.

The Assembly and Application of ‘Shear Rings’: A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

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