The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

Luke A. White; Emily V. Stevenson; J. Winny Yun; Randa Eshaq; Norman R. Harris; David K. Mills; Alireza Minagar; Pierre-Olivier Couraud; J. Steven Alexander

doi:10.3791/54632

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The Assembly and Application of ‘Shear Rings’: A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

JoVE Journal
Biology

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JoVE Journal Biology

The Assembly and Application of ‘Shear Rings’: A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

8,056 Views

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09:20 min

•

October 31, 2016

DOI:

10.3791/54632-v

DOI:

10.3791/54632-v

•

09:20 min

October 31, 2016

•

8041 Views

Luke A. White¹, Emily V. Stevenson¹, J. Winny Yun¹, Randa Eshaq¹, Norman R. Harris¹, David K. Mills², Alireza Minagar³, Pierre-Olivier Couraud⁴, J. Steven Alexander¹

¹Molecular and Cellular Physiology,Louisiana State University Health Sciences Center in Shreveport ²Biological Sciences,Louisiana Tech University ³Neurology,Louisiana State University Health Sciences Center in Shreveport ⁴Institut Cochin, Inserm U1016, Cnrs Umr8104,Université Paris Descartes

Summary

Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.