Intracellular Atp Measurement
Intracellular Atp Measurement - Related Videos
03:40
Imaging Intracellular ATP Dynamics Using FRET-Based Sensors in an Organotypic Mouse Hippocampal Slice
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2025
Source: Lerchundi, R., et. al. Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK. J. Vis. Exp. (2019)This video demonstrates the use of FRET(Fluorescence Resonance Energy Transfer)-based sensors to measure intracellular ATP levels in organotypic mouse hippocampal slices, enabling real-time visualization of ATP dynamics in neurons and astrocytes under physiological and experimental conditions.
08:32
The ATP Bioluminescence Assay
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2023
In fireflies, the luciferase enzyme converts a compound called luciferin into oxyluciferin, and produces light or “luminescence” as a result. This reaction requires energy derived from ATP in order to proceed, so researchers have exploited the luciferase-luciferin interaction to gauge ATP levels in cells. Given ATP’s role as the cell’s currency of energy, the ATP bioluminescence assay can provide insight into cellular metabolism and overall cell health.In this video, JoVE discusses cellular...
01:31
ATP Yield
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2019
Cellular respiration produces 30 - 32 ATP per glucose molecule. Although most of the ATP results from oxidative phosphorylation and the electron transport chain (ETC), 4 ATP are gained beforehand (2 from glycolysis and 2 from the citric acid cycle). The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
01:08
Hydrolysis of ATP
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2019
The bonds of adenosine triphosphate (ATP) can be broken through the addition of water, releasing one or two phosphate groups in an exergonic process called hydrolysis. This reaction liberates the energy in the bonds for use in the cell—for instance, to synthesize proteins from amino acids. If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine monophosphate—by the removal of a second...
02:56
An ATP Utilization Assay for Determining Bacterial Survival Under Antibiotics Treatment
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2025
This video describes the simple ATP-measuring assay using luciferase glow reagent. This assay is used to quantify Neisseria gonorrhoeae survival after treatment with ceftriaxone.
FAQs
Cellular ATP supplies the energy required during the luciferase-catalyzed oxidation of luciferin. This reaction produces light, and the emitted intensity is related to the amount of ATP present. A calibration step connects measured light levels with ATP concentration, allowing researchers to compare intracellular energy availability among samples or experimental conditions.
Calibration establishes how light intensity corresponds to ATP concentration rather than treating luminescence as an unquantified signal. With that relationship defined, researchers can interpret differences in emitted light as differences in intracellular ATP. This makes comparisons across treatments or biological conditions more meaningful when evaluating metabolic state, cell viability, or cytotoxicity.
Because intracellular ATP changes rapidly with cellular activity, measurements can reflect shifting metabolic responses rather than only a fixed property of a cell population. Changes in ATP may therefore help researchers assess how cells respond to treatments, disease-related conditions, or altered culture performance, particularly when results are compared across defined experimental conditions.
Researchers compare ATP levels across treatments or biological conditions to identify changes associated with cell viability, proliferation, metabolic responses, or cytotoxicity. A lower or higher measurement is interpreted in the context of the comparison, rather than alone. This approach helps connect an experimental treatment with changes in cellular energy status and overall cell behavior.
In cultured-cell studies, ATP measurements provide a quantitative readout for evaluating cell performance and responses to experimental conditions. Researchers can use the assay to examine proliferation, viability, metabolic effects, or cytotoxicity by comparing measurements among culture conditions. The resulting data support assessment of how treatments or other biological factors influence the cells.
Intracellular ATP measurement supports investigations of bioenergetics, disease mechanisms, drug effects, and cultured-cell performance. Its value comes from linking ATP levels with cellular energy availability and metabolic state, while comparisons across conditions reveal treatment-associated changes. The technique can therefore contribute both to mechanistic studies and to evaluating how cells respond to experimental interventions.