Fetal Liver Isolation
Fetal Liver Isolation - Related Videos
07:51
Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
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Cited by 44 •
2015
Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.
08:19
Isolation of Leukocytes from the Human Maternal-fetal Interface
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Cited by 57 •
2015
Described herein is a protocol to isolate and further study the infiltrating leukocytes of the decidua basalis and decidua parietalis - the human maternal-fetal interface. This protocol maintains the integrity of cell surface markers and yields enough viable cells for downstream applications as proven by flow cytometry analysis.
07:32
Porcine Normothermic Isolated Liver Perfusion
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Cited by 3 •
2023
The porcine model of liver normothermic machine perfusion (NMP), described here, can be successfully used to study NMP as a preservation strategy, a tool for viability assessment, and a platform for organ repair. It holds a high translational value, however it is technically challenging and labor-intensive.
12:06
Isolation of CD133+ Liver Stem Cells for Clonal Expansion
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Cited by 22 •
2011
Here we describe the isolation of CD133 expressing liver stem cells and cancer stem cells from whole murine liver, a process that requires tissue digestion, cell enrichment, and flow cytometry isolation. We include methods for advanced single cell isolation and clonal expansion.
07:39
Isolation of Rat Portal Fibroblasts by In situ Liver Perfusion
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Cited by 22 •
2012
A technique for isolating portal fibroblasts from rat liver is described. Livers are perfused and digested in situ with collagenase, followed by ex vivo digestion of the liver slurry and size selection of cells. This method provides a pure population of portal fibroblasts without the need for passage in culture.
FAQs
The preparation brings together developing hepatocytes, hematopoietic progenitors, and stromal populations, allowing their contributions to be examined within the same biological material. Researchers can therefore study liver maturation alongside blood-cell formation and cellular differentiation. This mixed composition is especially useful for investigating how distinct cell types relate to tissue-specific gene expression during development.
Dissociation provides two complementary preparation formats. Individual cells can support examination of cellular differentiation and population-specific properties, whereas small tissue fragments retain a more organized sample for studying liver material at the tissue scale. Choosing between these formats helps align the preparation with the intended culture or analysis system and the biological question.
Fetal liver isolation can reveal coordinated processes that extend beyond organ development, including hematopoiesis, the formation of blood cells, and differentiation of developing cell populations. It also supports examination of tissue-specific gene expression. Together, these measurements help connect cellular behavior with the mechanisms that shape both liver development and blood-cell formation.
Controlled laboratory handling is important because the isolated tissue must be separated from surrounding structures, processed into suitable cell or tissue formats, and maintained in an appropriate culture or analysis system. These conditions make the resulting preparation usable for consistent examination of development, differentiation, gene expression, and other cellular features.
The workflow begins with careful removal of the fetal liver from surrounding tissues. The recovered material is then dissociated into individual cells or small tissue fragments, depending on the planned investigation. Finally, the preparation is maintained in a suitable culture or analysis system so researchers can examine its cellular composition and developmental properties.
These preparations provide access to several classes of biological information, including the presence and behavior of developing hepatocytes, hematopoietic progenitors, and stromal populations. They can also support analysis of cellular differentiation and tissue-specific gene expression. The resulting data help characterize how developing liver tissue acquires its mature functions and organization.
The technique is useful when researchers need to examine developmental disorders, regeneration, or toxicology in relation to developing liver tissue. Isolated preparations provide material for studying cellular mechanisms rather than only observing the whole organ. This makes them relevant for connecting altered cell behavior or gene expression with broader developmental and tissue-level outcomes.
Fetal liver contains hematopoietic progenitors, so its isolation provides material for examining blood-cell formation during development. Studying these progenitors alongside hepatocytes and stromal populations can place hematopoiesis within the context of a developing organ. This links tissue biology with cellular differentiation and helps clarify how multiple developmental processes coexist in fetal liver.