Injection Syringe
Injection Syringe - Related Videos
09:58
Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology
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Cited by 31 •
2018
Mesh electronics probes seamlessly integrate and provide stable, long-term, single-neuron level recording within the brain. This protocol uses mesh electronics for in vivo experiments, involving the fabrication of mesh electronics, loading into needles, stereotaxic injection, input/output interfacing, recording experiments, and histology of tissue containing mesh probes.
02:28
Extraction of Apoplastic Bacteria Using a Syringe-Based Pressure Method
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2025
Source: Rufián, J. S., et al, Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a syringe-based pressure method for extracting apoplastic bacteria, which proliferate and form microcolonies within the apoplast, the intercellular space between plant cells. The method yields debris-free bacterial samples suitable for downstream single-cell analysis.
03:45
Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland
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2023
This video describes an intraductal injection technique where we deliver injection mix into the ducts of mouse mammary glands.
03:48
Mouse Bladder Wall Injection
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Cited by 34 •
2011
Mouse bladder wall injection is a useful approach to orthotopically study bladder stem cell and cancer biology. This delicate microsurgical method can be mastered with careful technique and practice.
09:19
Intraperitoneal Injection into Adult Zebrafish
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Cited by 238 •
2010
We demonstrate intraperitoneal injection into adult zebrafish. We use a 10 μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III. This demonstration includes the use of cold water as an anesthetic.
FAQs
Pressure differences created by plunger movement control fluid transfer. Pulling the plunger lowers pressure inside the barrel relative to the liquid outside, allowing liquid to enter through the needle. Pressing it forward reverses the pressure relationship and expels the contents. This mechanism makes plunger position central to controlled dosing, sample transfer, and dilution preparation.
Calibration markings translate plunger position into an intended liquid volume, so reading them consistently is essential for reproducible biology experiments. The scale supports measurement of reagents, samples, or dilution components rather than relying on approximate amounts. Inaccurate reading or volume selection can change concentrations and make experimental results difficult to compare.
Needle dimensions affect how the syringe performs during liquid delivery and sampling, and they are identified as factors influencing accuracy, safety, and reproducibility. Needle selection should therefore be considered alongside the intended task and target site. Treating needle choice as interchangeable across experiments can compromise controlled administration or reliable sample handling.
Sterile handling helps protect substances, samples, and target sites from unwanted contamination while supporting safe use. It is especially relevant when a syringe administers substances to experimental organisms or transfers biological samples. Because handling practices influence safety and reproducibility, maintaining sterility is part of controlling experimental conditions rather than merely a procedural preference.
Determine the required volume from the syringe’s calibrated markings, then draw the liquid by retracting the plunger and deliver it by pressing the plunger forward. Consistent volume readings, appropriate needle dimensions, and sterile handling help preserve accuracy and reproducibility when reagents or samples are combined for dilution preparation.
The device supports several distinct tasks: delivering reagents, administering substances to experimental organisms, transferring samples, and preparing precise dilutions. Its value is greatest when a procedure requires a measured liquid volume rather than an uncontrolled transfer. These uses connect the same pressure-driven tool to laboratory preparation, experimental treatment, and biological sampling.