Intracardiac Injection
Intracardiac Injection - Related Videos
08:36
Ultrasound Imaging-guided Intracardiac Injection to Develop a Mouse Model of Breast Cancer Brain Metastases Followed by Longitudinal MRI
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Cited by 26 •
2014
A breast cancer brain metastasis mouse model is established with ultrasound imaging-guided intracardiac injection of MDA-MB231/Br-GFP cells. Development of multifocal intracranial metastases has been monitored longitudinally using high-resolution 9.4 T MRI.
04:38
Intracardiac Delivery of Viral Vectors: A Technique to Inject Viral Vectors Intracardially to Study Pancreatic Metastasis in Murine Model
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2023
This video describes the protocol for intracardiac injection of retroviruses into the transgenic mouse to ensure gene transfer into proliferating pancreatic β premalignant lesions. This method can also help in assessing the functions of candidate genes in tumor metastasis.
07:45
Ultrasound-guided Intracardiac Injection of Human Mesenchymal Stem Cells to Increase Homing to the Intestine for Use in Murine Models of Experimental Inflammatory Bowel Diseases
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Cited by 7 •
2017
Murine studies in models of colonic inflammation have demonstrated that a small percentage (1 - 5%) of mesenchymal stem cells (MSC) injected intravenously or intraperitoneally home to the inflamed colon1,2. This study shows that ultrasound-guided intracardiac injections of MSCs result in increased localization to the intestine.
03:18
Stimulation of the Intracardiac Nervous System to Assess Susceptibility to Arrhythmia
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2025
Source: Jungen, C., et al. Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System. J. Vis. Exp. (2018).The video demonstrates an ex vivo technique for stimulating the intracardiac nervous system to assess the heart’s susceptibility to arrhythmia. Using a perfusion apparatus, an isolated mouse heart is perfused with an oxygenated buffer. The intracardiac nervous system is then stimulated at a high frequency to induce arrhythmias.
08:09
A Novel Ex Ovo Banding Technique to Alter Intracardiac Hemodynamics in an Embryonic Chicken System
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Cited by 2 •
2016
For the first time we present here a reproducible banding procedure to alter hemodynamics in the developing heart ex ovo. This is achieved by partially constricting the outflow tract (OFT).
FAQs
Placement determines whether the injected material enters the circulation or remains concentrated near cardiac tissue. Delivery into a heart chamber favors cardiovascular distribution, whereas placement in cardiac tissue can produce a more localized exposure. This distinction is important when interpreting tracer movement, drug effects, cell behavior, or treatment responses in animal models.
Distribution depends on the accuracy of anatomical targeting, the cardiac site selected, and local tissue conditions at the time of delivery. Controlled administration also affects how consistently material reaches the intended compartment. These variables can change whether results reflect systemic circulation, local cardiac exposure, or a combination of both.
Small differences in needle placement or delivery control can alter the destination and spread of the injected material. Consistent targeting helps researchers compare animals and distinguish biological effects from procedural variation. Careful control is therefore central to evaluating cardiovascular distribution, cardiac function, and therapeutic responses with greater confidence.
The technique can be used to administer experimental drugs, tracers, cells, and other test materials in biological studies. Each material may be examined for a different outcome, such as movement through the cardiovascular system, retention near cardiac tissue, effects on cardiac function, or response to a potential therapy.
A study first identifies the cardiac site appropriate for the experimental goal. The needle is then positioned through the chest wall at that target, and the material is delivered in a controlled manner. Subsequent evaluation examines distribution, cardiac function, or therapeutic response, depending on the material and research question.
Researchers may select this approach when they need direct access to a heart chamber or cardiac tissue and want to study cardiovascular distribution or localized cardiac effects. It can support experiments involving drugs, tracers, cells, or other test substances. Because the procedure affects both delivery and interpretation, ethical practice and careful targeting remain essential.