Nanofluidic Arrays
Nanofluidic Arrays - Related Videos
11:13
Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
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Cited by 1 •
2016
We propose a simple self-assembly technique of silica colloidal nanoparticles to create a nanofluidic junction between two microchannels in polydimethylsiloxane (PDMS). Using this technique, a nanoporous bead membrane with a pore size down to ~45 nm was built inside a microchannel and applied to electrokinetic preconcentration of DNA samples.
07:50
Chip-Based Digital PCR to Detect Rare Transcript Variants Using a Nanofluidic Chip
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2025
This video demonstrates chip-based digital PCR — a variation of the digital PCR technique that is useful in detecting rare transcript variants. The PCR reaction is partitioned into the chambers of a nanofluidic chip, each of which acts as an independent reaction. The detection of fluorescence signals from the chambers with amplified targets confirms the presence of rare transcript variants in the sample.
09:16
Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
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Cited by 7 •
2015
Array CGH for the detection of genomic copy number variants has replaced G-banded karyotype analysis. This paper describes the technology and its application in a diagnostic service laboratory.
07:32
Synthesis of Graphene Nanofluids with Controllable Flake Size Distributions
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2019
A method for synthesizing graphene nanofluids with controllable flake size distributions is presented.
19:40
Design and Use of Multiplexed Chemostat Arrays
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Cited by 108 •
2013
We developed and validated a small-footprint array of miniature chemostats built from readily available parts for low cost. Physiological and experimental evolution results were similar to larger volume chemostats. The ministat array provides a compact, inexpensive, and accessible platform for traditional chemostat experiments, functional genomics, and chemical screening applications.
FAQs
Nanoscale confinement makes surface interactions more influential and places channels, pores, or wells at dimensions comparable to individual molecules. This can alter how biomolecules move through the array and supports controlled separation, concentration, trapping, or detection. In biological experiments, the resulting molecular-scale control helps researchers examine individual DNA molecules and other biomolecular processes with high spatial resolution.
Pressure-driven transport moves samples through nanostructures using an applied pressure difference, whereas electrokinetic transport uses electrical forces to direct movement. Both approaches provide ways to control how fluids and biomolecules pass through patterned channels or pores. Selecting one transport mode can support the array’s intended function, including molecular separation, concentration, trapping, or detection.
The arrangement of channels, pores, and wells determines how samples encounter different regions of the nanostructure. Interconnected designs can coordinate fluid movement with localized molecular handling, allowing one system to combine transport with separation, concentration, trapping, or detection. Patterning many such structures in parallel also supports higher analytical throughput than examining a single nanoscale region alone.
A typical workflow begins by introducing a biological sample into a patterned array, followed by directing its movement through nanoscale channels, pores, or wells. Pressure-driven or electrokinetic transport then positions the fluid or biomolecules for separation, concentration, trapping, or detection. The resulting behavior is measured at a molecular scale suited to the biological question.
Nanofluidic arrays support several biology applications, including single-molecule analysis, DNA manipulation, and biomarker detection. Their nanoscale architecture can also help researchers investigate transport through membranes and cellular-scale barriers. These uses take advantage of controlled molecular movement and small sample volumes, enabling biological measurements that focus on individual molecules or confined transport processes.
These systems can provide information about how biomolecules move, separate, concentrate, become trapped, or are detected within confined nanostructures. In biology, that information can reveal properties of individual molecules, support DNA studies, identify biomarkers, and clarify transport through membrane-like or cellular-scale barriers. Parallel array designs further increase the number of measurements obtained from limited samples.