Ice-cap Method
Ice-cap Method - Related Videos
11:15
Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping
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Cited by 4 •
2011
The Ice-Cap method allows one to grow plants in 96-well plates and non-destructively harvest root tissue from each seedling. DNA extracted from this root tissue can be used for genotyping reactions. We have found that Ice-Cap works well for Arabidopsis thaliana, tomato, and rice seedlings.
11:00
Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis
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Cited by 8 •
2010
Animal caps overexpressing gene product(s) are transplanted to the flank of developing Xenopus laevis embryos in order to establish whether tissue is determined.
08:55
In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
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Cited by 13 •
2012
This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. The RNA is purified and a fraction of the uncapped RNA is enzymatically capped using the Vaccinia virus capping enzyme. In the final step, the mRNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity.
10:40
Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
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Cited by 3 •
2016
Here, we present human cell culture protocols to analyze translation initiation factors that bind the 5' cap of mRNA during physiological oxygen conditions. This method utilizes an Agarose-linked m7GTP cap analog and is suitable to investigate cap-binding factors and their interacting partners.
07:27
Producing, Characterizing and Quantifying Biochar in the Woods Using Portable Flame Cap Kilns
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Cited by 5 •
2024
New methods for disposing of forestry slash piles in place produce pyrogenic carbon for restoring forest soil health and for carbon removal and sequestration. Here, we present a biochar production method integrating a new carbon removal accounting methodology and a digital application.
FAQs
Melting ice absorbs heat from the surrounding specimen and environment, helping maintain near-freezing conditions rather than allowing temperature to rise. This reduces molecular motion and slows temperature-related changes while the material is being handled or processed. The cooling effect can therefore help preserve the specimen’s condition long enough to support more consistent laboratory work.
Lower temperatures reduce molecular motion and enzymatic activity, which can slow processes that contribute to specimen degradation. This is particularly relevant when cells or tissues must remain stable during handling, microscopy, or dissection. The benefit is temporary rather than absolute, because the specimen still requires controlled exposure and may be harmed if freezing or ice formation occurs.
Protection depends mainly on exposure time, temperature, and the degree of specimen contact with the ice. Controlled conditions can limit degradation, whereas prolonged exposure or unwanted ice formation may damage cellular structures. Monitoring these variables helps maintain cooling without crossing from near-freezing stabilization into conditions that compromise the biological material.
The method is intended to provide controlled, near-freezing cooling rather than prolonged freezing of the specimen. That distinction matters because cooling can reduce enzymatic activity and temperature-related change, while ice formation within or around biological material can damage cellular structures. Researchers therefore need to manage contact and exposure so stabilization does not become harmful freezing.
A suitable procedure centers on applying a controlled ice layer while the specimen is being handled or processed, then limiting exposure to the time needed for stabilization. Temperature and specimen contact should remain controlled throughout the procedure. These precautions help preserve the material during microscopy, dissection, or related work without encouraging prolonged freezing or structural damage.
The ice-cap method can support short-term preservation when biological specimens must remain stable during handling or processing. Its stated uses include microscopy and dissection, as well as other laboratory procedures in which temperature-related changes could reduce consistency. It is most useful when temporary cooling is needed and the specimen can be monitored for excessive exposure or ice formation.
Careful use can minimize degradation and improve consistency across handling or processing steps by limiting temperature-related changes. The method may help maintain the condition of cells or tissues long enough for microscopy, dissection, or other procedures. Outcomes depend on controlling the cooling conditions, because excessive duration or ice formation can instead compromise cellular structure.