Cecal Injection
Cecal Injection - Related Videos
11:53
Cecal Ligation Puncture Procedure
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Cited by 200 •
2011
The mouse model of cecal ligation and puncture as a valuable tool for the study of human sepsis.
06:40
Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice
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Cited by 56 •
2014
Experimental sepsis can be induced in mice using the cecal ligation and puncture (CLP) method. Current protocols to assess autophagy in vivo in the context of CLP-induced sepsis are presented here: A protocol for measuring autophagy using (GFP)-LC3 mice, and a protocol for measuring autophagosome formation by electron microscopy.
04:08
Intracecal Tumor Cell Injection: A Technique to Deliver Cancer Cells for Establishing Orthotopic Colorectal Cancer Mouse Model
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2023
The video describes the technique for intracecal injection of cancerous cells to develop an orthotopic mouse model with highly uniform tumors. Therefore, the orthotopic mouse model allows realistic simulation of metastasis, providing a valuable tool to study the phenotype changes during tumor growth and dissemination.
07:30
Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
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Cited by 7 •
2019
This protocol describes techniques to measure infectious outcomes underlying secondary hospital-acquired infections in the immunosuppressive condition, first by establishing cecal ligation/puncture mice then challenging them with intranasal infection to create a clinically relevant model of immunosuppression sepsis.
05:28
Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis
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Cited by 3 •
2022
This protocol presents the operative details of cecal ligation and puncture (CLP) in a mouse model of sepsis. CLP is one of the most widely used techniques to create an animal model of sepsis. Therefore, a standardized CLP protocol is required for the attainment of reliable research results.
FAQs
Its main experimental advantage is placing the developing lesion in the cecum rather than in a nonnative site. Tumor cells can interact with surrounding stromal, vascular, and immune components in the tissue where colorectal tumors arise. These local interactions provide context for examining tumor behavior and treatment effects under conditions that more closely resemble the primary tumor environment.
Placement within or beneath the cecal wall helps establish a localized tumor at the intended tissue site. This positioning supports interaction with nearby tissue structures and can influence how the lesion develops, invades, and progresses. A controlled injection is therefore important for creating a reproducible model in which local tumor biology can be studied in vivo.
The resulting model can support studies of tumor growth, invasion, metastasis, and response to treatment. Because the lesion develops in the cecum, researchers can examine these processes alongside local stromal, vascular, and immune influences. This makes the technique useful for connecting cancer mechanisms with disease progression and therapeutic outcomes in an animal model.
Ectopic models place tumor material outside its native tissue environment, whereas Cecal Injection establishes the experimental lesion in the cecum. The latter preserves local tissue relationships that may affect growth, invasion, metastasis, and treatment response. Consequently, it offers a site-specific approach for colorectal cancer research when the primary tumor setting is scientifically important.
The procedure begins by surgically exposing the cecum. Researchers then deliver a controlled injection containing tumor cells or another experimental material into or beneath the cecal wall. After placement, the model is followed in vivo to examine tumor establishment and subsequent outcomes such as progression, invasion, metastasis, or response to an intervention.
The injected material may consist of tumor cells or other experimental materials, depending on the research question. The material is delivered in a controlled manner and positioned within or beneath the cecal wall. This flexibility allows investigators to use the same tissue-site approach for different studies of colorectal cancer mechanisms, progression, or therapeutic strategies.
Researchers would choose this approach when they need an in vivo colorectal cancer model that reflects the tumor’s native tissue setting. It is particularly relevant for evaluating mechanisms of disease progression, interactions with surrounding tissue components, therapeutic strategies, and treatment response. The method is most informative when tissue location is central to the experimental question.
Following tumor establishment, investigators can assess how the lesion grows and progresses within the cecum, including evidence of invasion or metastasis. They can also compare responses to experimental treatments. These observations help connect tumor behavior with the local stromal, vascular, and immune context and support evaluation of cancer mechanisms and therapeutic effectiveness.