Journal
/
Immunology and Infection
/
Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

DOI:
10.3791/63584-v

•

05:28 min

•

December 09, 2022

•

, , , , ,
1Department of Vascular Surgery, Xiangya Hospital,Central South University

Chapters

  • 00:14Introduction
  • 00:49Preparation
  • 01:15Operation
  • 03:53Treatment
  • 04:52Representative Results
  • 05:16Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents the operative details of cecal ligation and puncture (CLP) in a mouse model of sepsis. CLP is one of the most widely used techniques to create an animal model of sepsis. Therefore, a standardized CLP protocol is required for the attainment of reliable research results.

Tags

Keywords: Cecal Ligation And PunctureCLPSepsisMouse ModelBiomarkerCitrullinated Histone H3CitH3NETosisC57BL/6 MiceAbdominal SurgeryAnesthesiaKetoprofenEuthanasia

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63584?article_type=v

Colon Ascendens Stent Peritonitis (CASP) – a Standardized Model for Polymicrobial Abdominal Sepsis
check_url/63584?article_type=v

Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice
check_url/63584?article_type=v

Influenza A Virus Studies in a Mouse Model of Infection
check_url/63584?article_type=v

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis
check_url/63584?article_type=v

Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
check_url/63584?article_type=v

Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
check_url/63584?article_type=v

A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
check_url/63584?article_type=v

A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model
check_url/63584?article_type=v

A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia
check_url/63584?article_type=v

A Mouse Ear Model for Allergic Contact Dermatitis Evaluation

Read Article