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Immunology and Infection
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JoVE Journal Immunology and Infection
Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis
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Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

Article DOI: 10.3791/63584-v • 05:28 min • December 9th, 2022
December 9th, 2022 •
1, 1, 1, 1, 1, 1
1Department of Vascular Surgery, Xiangya Hospital, Central South University

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This protocol presents the operative details of cecal ligation and puncture (CLP) in a mouse model of sepsis. CLP is one of the most widely used techniques to create an animal model of sepsis. Therefore, a standardized CLP protocol is required for the attainment of reliable research results.

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Mouse Model Cecal Ligation And Puncture Sepsis Blood Biomarker Citrullinated Histone H3 NETosis Diagnostic Biomarker Male C57BL/6 Mice Anesthesia Depilatory Cream Sterile Surgical Instruments Iodine Disinfection Abdominal Incision Cecum Isolation Silk Suture Ligation Severity Of Sepsis
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