Orthotopic Engraftment
Orthotopic Engraftment - Related Videos
09:02
Pre-Conditioning the Airways of Mice with Bleomycin Increases the Efficiency of Orthotopic Lung Cancer Cell Engraftment
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Cited by 8 •
2018
We describe a method to significantly enhance orthotopic engraftment of lung cancer cells into the murine lungs by pre-conditioning the airways with injury. This approach may also be applied to study stromal interactions within the lung microenvironment, metastatic dissemination, lung cancer co-morbidities, and to more efficiently generate patient derived xenografts.
09:16
Orthotopic Small Bowel Transplantation in Rats
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Cited by 4 •
2012
Small bowel transplantation has become an accepted treatment option for patients with irreversible intestinal failure. Our experimental model of orthotopic small bowel transplantation in rats serves as a reliable tool to address underlying immunologic and inflammatory processes that complicate intestinal transplantation.
04:36
Adenocarcinoma Engraftment: A Method to Implant Cancer Cells into the Murine Lungs
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2023
This video describes a method to engraft lung adenocarcinoma cells in murine lungs by preconditioning the airways with fibrosis-inducing agent–bleomycin. This method significantly enhances orthotopic engraftment of lung cancer cells into murine...
03:53
An Orthotopic Sciatic Nerve Xenograft for Neurofibromatosis Type 1 Neurofibromas
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2025
Neurofibromatosis type 1 (NF1) leads to benign peripheral nerve sheath tumors (neurofibromas) that require a specific nerve microenvironment for growth. An optimized orthotopic xenograft model was developed, using intraneural injection of human Schwann cells into NSG mice. This model recapitulates human neurofibromas and provides a valuable platform to evaluate therapeutic strategies.
10:52
Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
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Cited by 1 •
2018
Mantle cell lymphoma (MCL) is a difficult to treat B cell disorder and it is equally difficult to establish a xenograft mouse model of primary MCL to study and develop therapeutics. Here, we describe the successful establishment of MCL xenografts in mice to help understand its underlying biology.
FAQs
The implanted tumor interacts with the organ’s native stroma, vasculature, extracellular matrix, and local signaling environment. These interactions help determine whether the tumor becomes established and how it progresses within that tissue. Because the model preserves these site-specific relationships, investigators can examine tumor behavior in an anatomical setting that more closely reflects the corresponding disease in patients.
The native organ provides tissue-specific conditions that can affect tumor biology, local invasion, metastasis, and treatment response. Its structural and signaling features may produce effects that are not represented when tumors grow at nonnative sites. Maintaining this local context therefore helps researchers distinguish responses associated with the tumor itself from those shaped by its surrounding tissue.
Orthotopic engraftment places cancer cells or tumor tissue in the anatomically corresponding organ, whereas a nonnative-site model does not preserve that organ-specific setting. The orthotopic approach retains interactions with local stroma, vasculature, extracellular matrix, and signaling factors. As a result, it can reveal tissue-specific tumor behavior and therapeutic effects that nonnative models may miss.
Orthotopic xenograft models and patient-derived tumor models provide complementary ways to investigate cancer in an organ-matched setting. Both can support studies of tumor establishment, progression, local invasion, metastasis, and therapeutic response. Their value comes from combining the implanted tumor material with the native tissue context, allowing researchers to evaluate behavior under more disease-relevant anatomical conditions.
A basic workflow begins by selecting cancer cells or tumor tissue and implanting that material into the corresponding organ of a model organism. The engrafted material is then studied as it interacts with the organ’s native microenvironment and progresses over time. Researchers can subsequently evaluate tumor biology, invasion, metastasis, or response to an anticancer treatment.
Researchers may choose this approach when the anatomical context could influence the question being studied. It is particularly relevant for examining local invasion, metastasis, tumor progression, and therapeutic response. The models also support preclinical evaluation of anticancer treatments by capturing tissue-specific effects that might not appear when tumors are established outside their corresponding organ.