Transillumination System
Transillumination System - Related Videos
09:59
Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses
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Cited by 18 •
2020
This protocol describes transillumination-assisted microdissection of segments of adult mouse seminiferous tubules representing specific stages of seminiferous epithelial cycle, and cell types therein, and subsequent immunostaining of squash preparations and intact tubule segments.
09:20
Isolation of Subcellular Ribosome Subpopulations Based on Recombinant Peptide Tag-Specific Location-Restricted Illumination-Enhanced Biotinylation
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2026
AviTag-specific Location-restricted Illumination-enhanced Biotinylation (ALIBi) is a novel optogenetically activated method developed for labeling and affinity-purifying subcellular populations of ribosomes for downstream sequencing and proteomic analysis.
05:26
Reporter Enzyme Fluorescence Imaging for In Vivo Tracking of Bacterial Infection
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2025
This video demonstrates the reporter enzyme fluorescence (REF) imaging for in vivo detection of Mycobacterium tuberculosis infection. The infected mice are intraperitoneally injected with a substrate for REF imaging. Upon being hydrolyzed by bacterial β-lactamase enzyme, the substrate emits fluorescence, the detection of which aids evaluation of infection.
16:44
Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging
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Cited by 15 •
2009
We suggest a Born normalized approach for Optical Projection Tomography (BnOPT) that accounts for the absorption properties of imaged samples to obtain accurate and quantitative fluorescence tomographic reconstructions. We use the proposed algorithm to reconstruct the fluorescence molecular probe distribution within small animal organs.
03:09
Fluorescence Molecular Tomography: An Imaging Technique for In Vivo Imaging of Fluorescent Protein-tagged Glioblastoma Xenografts in Mouse Model
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2025
In this video, we describe fluorescence molecular tomography or FMT for in vivo imaging of fluorescent protein-tagged glioblastoma xenografts in mouse models. The technique is useful for elucidating the effects of anti-tumor therapeutics at the molecular level.
FAQs
Contrast depends on how much light different regions absorb, scatter, or transmit. Variations in specimen thickness and optical density cause some areas to appear lighter or darker than neighboring structures. These differences make subtle anatomical or pathological features more visible, allowing researchers to distinguish tissue organization, vessels, lesions, or changes within developing tumor models.
The detected signal reflects both light that passes through the specimen and light redirected by structures within it. Their combined intensity creates the image pattern rather than a uniform illumination field. Interpreting these differences helps researchers relate visible contrast to specimen organization and identify structural features without requiring a fluorescent label.
Transillumination is particularly useful when the main objective is structural information rather than detection of a fluorescent signal. Because it provides rapid, label-free visualization, researchers can examine morphology without relying on fluorescent labeling. It therefore complements fluorescence microscopy when tissue organization, vessels, lesions, or overall changes are the primary outcomes.
Place the specimen between an illumination source and the optical collection side, then position the system so transmitted and scattered light can be recorded across the sample. Adjust the arrangement to reveal differences in brightness produced by the specimen. The resulting image can then document visible morphology and structural variation.
In cancer research, the method can visualize tissue organization, blood vessels, lesions, and developing tumor models. Researchers may use these images to document morphology and evaluate experimental changes over time or between conditions. Its label-free operation provides structural information that can complement other microscopy approaches used to investigate biological samples.
The principal outcome is a visual record of specimen structure and morphology. Image contrast can reveal organization, vascular features, lesions, or changes in a developing tumor model. These observations help researchers compare experimental states and assess structural effects, while fluorescence or other microscopy methods can provide complementary information when needed.