Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

Claudio Vinegoni; Daniel Razansky; Jose-Luiz Figueiredo; Lyuba Fexon; Misha Pivovarov; Matthias Nahrendorf; Vasilis Ntziachristos; Ralph Weissleder

doi:10.3791/1389

Journal

/

Biology

/

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

10,285 Views

•

16:44 min

•

June 02, 2009

DOI:

10.3791/1389-v

DOI:

10.3791/1389-v

•

16:44 min

June 02, 2009

•

2 Views

Claudio Vinegoni^1,2, Daniel Razansky³, Jose-Luiz Figueiredo^1,2, Lyuba Fexon^1,2, Misha Pivovarov^1,2, Matthias Nahrendorf^1,2, Vasilis Ntziachristos³, Ralph Weissleder^1,2

¹Center for Systems Biology,Harvard Medical School, ²Center for Systems Biology,MGH – Massachusetts General Hospital, ³Institute for Biological and Medical Imaging,Technical University of Munich and Helmholtz Center Munich

Summary

We suggest a Born normalized approach for Optical Projection Tomography (BnOPT) that accounts for the absorption properties of imaged samples to obtain accurate and quantitative fluorescence tomographic reconstructions. We use the proposed algorithm to reconstruct the fluorescence molecular probe distribution within small animal organs.