Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

In order to perform normalized born optical projection tomography, the sample is first fixed and embedded in a cylinder of auger. The sample is then rotated along a vertical axis and then uniformly illuminated with an excitation wavelength, and the transmission is measured with a C, CD suitable optics. The fluorescent signal at wavelength is measured in transillumination mode.

The acquired fluorescent and absorption images are used to determine the normalized born ratio and reconstruct the distribution of fluoro fours within the sample. Hi, my name is Claudia Naone and I work at the Centers for Systems Biology at Mass General Hospital, Harvard Medical School. I'm Danielle Raki from the Institute for Biological and Medical Imaging at the Technical University of Munich and Al Center Munich.