Wastewater Purification

Wastewater purification is the treatment of contaminated water to remove suspended solids, dissolved chemicals, nutrients, pathogens, and other pollutants before reuse or release, protecting ecosystems and public health. It works through complementary physical, chemical, and biological processes: screening and settling separate particles, coagulants destabilize colloids so they form removable flocs, and oxidation, adsorption, or microbial reactions transform or capture dissolved contaminants. In chemistry, these processes depend on properties such as solubility, surface charge, pH, and redox potential, which guide treatment design and performance. Purified water can support safe discharge, industrial reuse, and resource recovery while reducing environmental pollution.

Wastewater Purification - Related Videos

Research

JoVE Journal - Environment

Comparison of Scale in a Photosynthetic Reactor System for Algal Remediation of Wastewater

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Cited by 3 •

2017

An experimental methodology is presented to compare the performance of small (100 L) and large (1,000 L) scale reactors designed for algae remediation of landfill wastewater. System characteristics, including surface area to volume ratio, retention time, biomass density, and wastewater feed concentrations, can be adjusted based on application.

Research

JoVE Journal - Environment
Free Sample

Use of a Battery of Chemical and Ecotoxicological Methods for the Assessment of the Efficacy of Wastewater Treatment Processes to Remove Estrogenic Potency

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Cited by 7 •

2016

Endocrine Disrupting Compounds (EDC) pose a substantial risk to the aquatic environment. Municipal wastewater treatment plants are major contributors to the estrogenic potency of surface waters. The methodology provided in this paper allows for an assessment of the efficacy and suitability of wastewater treatment processes with respect to EDC removal.

Education

JoVE Core - Microbiology

Microbial Wastewater Treatment

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2026

Microbial communities in aquatic ecosystems play a key role in the natural breakdown of contaminants introduced through domestic and industrial effluents. Acting as biological catalysts, these microbes change and mineralize a wide range of organic and inorganic pollutants under different redox conditions.In oxygen-rich surface waters, aerobic heterotrophs lead organic matter breakdown, using oxygen as the terminal electron acceptor to efficiently oxidize substrates to carbon dioxide and water.

Plasmid Purification

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2023

Plasmid purification is a technique used to isolate and purify plasmid DNA from genomic DNA, proteins, ribosomes, and the bacterial cell wall. A plasmid is a small, circular, double-stranded DNA that is used as a carrier of specific DNA molecules. When introduced into a host organism via transformation, a plasmid will be replicated, creating numerous copies of the DNA fragment under study. In this video, a step-by-step generalized procedure is described for how to perform plasmid purification.

Gel Purification

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2023

Gel purification is used to recover DNA fragments after electrophoretic separation. DNA recovery from an agarose gel includes three basic steps: binding, washing and eluting from a silica column. DNA is believed to bind to silica in the presence of high salt via a salt bridge. Following binding, DNA is washed of impurities and eluted under low salt conditions disrupting this interaction. This video goes through a step-by-step, generalized procedure for cutting out a band from the gel, gel...

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