Medaka Fish Model
Medaka Fish Model - Related Videos
08:53
Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model
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Cited by 10 •
2017
Small laboratory fish have become popular models for bone research on the mechanisms underlying human bone disorders and for the screening of bone-modulating drugs. In this report, we describe a protocol to assess the effect of alendronate on bone cells in medaka larvae with osteoporotic lesions.
07:25
Microinjection of Medaka Embryos for use as a Model Genetic Organism
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Cited by 26 •
2010
Medaka and zebrafish are complementary for genetic dissection of vertebrate genome functions. This protocol highlights the key points for successful microinjection into medaka embryos, an important technique for embryological and genetic analysis using medaka and zebrafish in a laboratory.
06:29
Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs
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Cited by 2 •
2016
Embryonic stages are the most susceptible to xenobiotics. Although chemical toxicity depends on salinity, no method exists to test the salinity dependence of toxicity to aquatic organisms. Here, we describe a new and high-throughput method for determining the salinity dependence of toxicity to aquatic embryos.
07:25
Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria
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Cited by 2 •
2017
A series of basic methods to enable the study of the reproductive ecology of fish kept in aquaria are described. These are useful protocols for collecting fish using SCUBA, transporting live fish, and observing the reproductive behavior of wild-caught fish kept in aquaria.
02:32
Osmoregulation in Fishes
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2019
When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments? Fish employ osmoregulatory strategies to balance bodily levels of water and dissolved ions (i.e., solutes), such as sodium and chloride. Imagine two solutions separated by a membrane that is permeable to water.
FAQs
Researchers can alter or examine genes and then follow resulting changes as embryos form tissues and organs. Because development is rapid and embryos remain optically accessible, investigators can connect a genetic change with effects on cell fate, body patterning, or organogenesis. This links molecular gene activity to visible developmental outcomes in a vertebrate context.
External fertilization makes embryos available for observation and experimental treatment without accessing an internal reproductive tract. Optical accessibility then allows tissue formation and organ development to be followed as they occur. Together, these features support direct examination of embryonic changes and make it easier to relate developmental timing to experimental conditions.
Medaka embryos provide a way to examine how cells acquire particular identities and become arranged into organized body structures. Researchers can observe tissue formation and organ development while testing how genetic changes or controlled environmental treatments alter those processes. The resulting observations help connect early patterning events with later anatomical outcomes in vertebrate development.
A typical study begins with established breeding to obtain externally fertilized embryos, followed by genetic manipulation, imaging, or a controlled environmental treatment, depending on the question. Investigators then observe tissue and organ development and compare outcomes across conditions. This workflow connects an experimental intervention with changes in embryonic development, gene function, or disease-related biology.
Medaka studies can combine breeding methods with genetic manipulation, optical imaging, and controlled environmental treatments. Breeding supplies experimental embryos, imaging documents developmental changes, and manipulation or treatment creates the condition being tested. Using these approaches together allows researchers to examine both normal development and how altered genetic or environmental conditions influence tissues and organs.
The model is useful when investigators need to relate vertebrate development to disease-related biology or regeneration. Its embryos support analysis of how genetic or environmental changes affect tissues and organs, while the broader organism provides developmental context. These studies can identify conserved mechanisms that connect normal formation, abnormal development, and regeneration processes.