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Chapters
- 00:05Title
- 00:55Introduction
- 01:28Mating and Egg Collection
- 02:58Making Agarose Plates for Holding Embryos
- 03:58Microinjection of Medaka Embryos
- 06:14Representative Images of Microinjected Embryos
- 06:59Conclusion
Medaka and zebrafish are complementary for genetic dissection of vertebrate genome functions. This protocol highlights the key points for successful microinjection into medaka embryos, an important technique for embryological and genetic analysis using medaka and zebrafish in a laboratory.
Tags
MicroinjectionMedaka EmbryosModel Genetic OrganismVertebrate Model OrganismsGenetic AnalysisEmbryological AnalysisPhenotype-driven Mutant ScreensZebrafishMouseFunctional OverlapNovel PhenotypesGenetic DissectionVertebrate Genome FunctionsCell-tracersLabeling CellsMRNAAnti-sense OligonucleotidesGene ExpressionTransgenic Lines
