Aggregate Disassembly
Aggregate Disassembly - Related Videos
02:49
Detection of Biofilm Disassembly Through a Dispersion Assay
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2025
The video showcases a biofilm dispersion assay using a multi-well plate. Buffer and glucose treatment disassemble the biofilm, releasing bacteria, while bile salts induce stress, retaining bacteria in the protective extracellular polymeric substance. Higher dispersion with buffer and glucose confirms successful biofilm disassembly, estimated by colony forming units measurement.
01:35
Disassembly of Intermediate Filaments
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2023
Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs. Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
07:24
In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation
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Cited by 4 •
2016
Disassembly of influenza A virus cores during virus entry into host cells is a multistep process. We describe an in vitro method to analyze the early stages of viral uncoating. In this approach, velocity gradient centrifugation is used to biochemically dissect the steps that initiate uncoating under defined conditions.
02:42
In Vitro Disassembly of the Influenza A Virus Core Using pH-Controlled Gradient Centrifugation
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2026
Source: Stauffer, S. et al. In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation. J. Vis. Exp. (2016)This video demonstrates the pH-dependent disassembly of influenza A virus (IAV) cores using gradient ultracentrifugation. It outlines glycerol gradient preparation, ultracentrifugation, and how different pH conditions affect IAV core disassembly.
08:26
Synthesis of Zeolites Using the ADOR (Assembly-Disassembly-Organization-Reassembly) Route
0 Views •
Cited by 6 •
2016
A protocol for the preparation of novel zeolites by the ADOR (Assembly-Disassembly-Organization-Reassembly) synthetic route is presented.
FAQs
Disassembly depends on disrupting the forces that hold a cluster together. These may include extracellular polymeric substances surrounding microbial communities, direct protein contacts, or cell-adhesion interactions. Because different aggregates rely on different cohesive components, the relevant disruption mechanism can influence whether the result is partial breakup, smaller units, or broader loss of organization.
Enzymatic degradation can break down extracellular polymeric substances or other molecular structures that stabilize an aggregate, while chemical changes can weaken protein contacts or adhesion. These mechanisms act on cohesion rather than treating all aggregates identically. Examining which interaction is affected helps researchers connect a physical change in organization with altered microbial persistence or immune access.
Fluid shear applies a physical force that can weaken or separate cohesive structures, whereas host-derived factors may alter aggregates through biologically active effects. Chemical changes and enzymatic degradation provide additional routes. Comparing these influences helps researchers determine whether breakup results from mechanical stress, molecular destabilization, or immune-environment effects that reshape microbial communities.
Not necessarily. The process describes a change in organization, such as breakup into smaller units, rather than direct evidence that the component cells or particles have been eliminated. This distinction matters in infection research because disassembly may improve antimicrobial penetration or phagocytic access, while persistence and clearance remain separate outcomes to evaluate.
A study can first characterize an organized cell, particle, or biomolecular cluster, then examine how a selected condition affects its cohesion. Researchers can evaluate the resulting smaller units alongside antimicrobial penetration, phagocytic clearance, or inflammatory signaling. This workflow links structural disruption with functional consequences instead of treating visible breakup as the only outcome.
It is especially relevant when researchers need to understand how biofilm structure affects persistence and access by antimicrobial agents or immune cells. Assessing disruption can reveal whether weakening extracellular polymeric substances changes the organization of the community. The resulting information supports investigation of strategies for treating infections that remain difficult to clear.
Breaking organized microbial communities into smaller units can change how immune components encounter the material and may influence phagocytic clearance. The process is also useful for examining inflammatory signaling associated with altered microbial organization. In this context, disassembly provides a way to study how infection environments modify both physical accessibility and immune responses.