Double Antigen Sandwich
Double Antigen Sandwich - Related Videos
03:33
Rapid Detection of Fecal Antigen of Helicobacter pylori Infection Based on Double Antibody Sandwich Detection Technology
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2025
In this study, a sandwich detection technique based on double antibodies was developed for rapid detection of fecal antigens infected with Helicobacter pylori.
14:22
ELISA Assays: Indirect, Sandwich, and Competitive
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2023
Source: Whitney Swanson1,2, Frances V. Sjaastad2,3, and Thomas S. Griffith1,2,3,4 1 Department of Urology, University of Minnesota, Minneapolis, MN 55455 2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 3 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455 4 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 Enzyme-linked immunosorbent assay (ELISA) is frequently used to measure the presence...
06:50
Sandwich-like Microenvironments to Harness Cell/Material Interactions
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Cited by 3 •
2015
The following protocol describes the procedure to assemble sandwich-like cultures to be used as an intermediate stage between bi-dimensional (2D) and three-dimensional (3D) cellular environments. The engineered systems can have applications in microscopy, biomechanics, biochemistry and cell biology assays.
10:44
Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
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Cited by 3 •
2017
We demonstrate a snap chip technology for performing cross-reactivity-free multiplexed sandwich immunoassays by simply snapping two slides. A snap apparatus is used for reliably transferring reagents from microarray-to-microarray. The snap chip can be used for any biochemical reactions requiring colocalization of different reagents without cross-contamination.
05:42
Using a Sandwich ELISA to Determine the Immunogenic Glycoprotein Content in a Vaccine
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2025
This video showcases an indirect enzyme-linked immunosorbent assay or ELISA sandwich immunocapture assay designed to assess immunogenic glycoprotein levels in vaccines. The assay plate, containing glycoprotein-specific antibodies, is incubated with serially diluted vaccine samples, including reference and test vaccines, followed by immunoassay to quantify glycoprotein content in the test vaccine.
FAQs
The two antigen copies create a capture arrangement in which patient antibodies first bind antigen on the plate and then interact with the labeled antigen. This links the measured signal to antibodies that recognize the same antigen, rather than to an independently targeted immunoglobulin class. The format therefore supports assessment of pathogen-related immune responses.
Signal development occurs after the plate is washed and a substrate is added to the enzyme label. Washing separates unbound components before color formation, while the resulting color reflects the amount of antigen-specific antibody captured between the two antigen molecules. Consequently, the assay can provide an indication of antibody concentration in the tested sample.
Its principal distinction is the detection reagent. Instead of using a secondary antibody directed against patient immunoglobulins, the assay uses a second copy of the relevant antigen carrying an enzyme label. Detection therefore depends on the patient's antibodies recognizing the antigen, while avoiding reliance on a separate anti-immunoglobulin antibody for signal generation.
A typical workflow immobilizes one antigen on a plate, exposes it to the patient sample, and allows antigen-specific antibodies to bind. An enzyme-labeled form of the same antigen is then added to attach to the captured antibodies. After washing away unbound material, substrate is added, and the developed color is interpreted in relation to antibody concentration.
Measuring total antigen-specific antibodies provides an overall indication of whether antibodies recognizing a particular antigen are present, without depending on a secondary anti-immunoglobulin reagent. This makes the format useful when the objective is to assess the broader antigen-specific antibody response rather than focus on a separately targeted immunoglobulin category.
The format can support serological diagnosis and surveillance of exposure by detecting antibodies associated with a pathogen-specific response. It also helps assess vaccine responses and evaluate pathogen-specific immunity. These applications make the assay relevant for examining immune responses across diagnostic, monitoring, and vaccine-related investigations.