Fluorescent Cell Tracking
Fluorescent Cell Tracking - Related Videos
08:39
Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy
0 Views •
2025
This article provides a detailed description of how to create samples for single-protein tracking in solid-supported lipid bilayers. It also explains a straightforward fluorescence microscope with single-molecule sensitivity and a fast frame rate. Finally, we outline the procedure for extracting single-protein trajectories.
03:27
Microfluidic Patterning and Fluorescence-Based Tracking of Single-Cell Bacterial Growth
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2025
Source: Pioli, R., et al., Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly. J. Vis. Exp. (2021)This video demonstrates the method of capturing single fluorescently tagged Escherichia coli (E. coli) cells in microfluidic traps using a carbon-free suspension and controlled flow, followed by nutrient addition to trigger growth and fluorescence imaging of microcolonies.
03:49
A Luciferase-Fluorescent Reporter Influenza Virus to Track Viral Infections
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2025
This video demonstrates a method to track viral infections in mouse models using a recombinant luciferase and fluorescence protein-expressing bi-reporter Influenza A virus. This method enables both in vivo and ex vivo studies, enabling researchers to gain insights into viral dynamics and the effects of interventions.
17:08
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
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Cited by 11 •
2014
Combinatorial 5 fluorescent proteins marking of hematopoietic stem and progenitor cells allows in vivo clonal tracking via confocal and two-photon microscopy, providing insights into bone marrow hematopoietic architecture during regeneration. This method allows non-invasive fate mapping of spectrally-coded HSPCs-derived cells in intact tissues for extensive periods of time following transplantation.
07:19
Cell Tracking Using Photoconvertible Proteins During Zebrafish Development
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Cited by 17 •
2012
Here, we present a method for the photoactivated switch of photoconvertible fluorescent proteins (PCFPs) in the living zebrafish embryo and further tracking of photoconverted protein at specific time points during development. This methodology allows monitoring of cell biological events underlying different developmental processes in a live vertebrate organism.
FAQs
A fluorophore absorbs light at an appropriate excitation wavelength and then emits light at a longer wavelength. Microscopy systems detect this emitted signal and record it over time. This difference between excitation and emission allows labeled cells to be distinguished during imaging, supporting measurements of their location, movement, survival, and interactions without relying only on endpoint observations.
Fluorescent dyes and genetically encoded markers provide two labeling approaches for living cells. Dyes directly tag cells, whereas genetically encoded markers create fluorescence through an introduced marker within the cell. Both approaches can support repeated observation, but the choice determines how the fluorescent label is associated with the cell during studies of migration, survival, or cellular interactions.
Time-lapse measurements show how cellular behavior changes rather than providing only a single snapshot. By recording images over time, researchers can quantify changes in cell location, movement, survival, and interactions. These measurements help connect dynamic behavior with disease progression or treatment responses, making it possible to examine when cellular events occur and how they relate to one another.
A typical workflow begins by labeling living cells with a fluorescent dye or genetically encoded marker. The labeled cells are then illuminated at the appropriate excitation wavelength, while a microscopy system detects the emitted light at a longer wavelength. Repeated imaging produces time-lapse records that can be analyzed for changes in location, movement, survival, and interactions.
In immunology and infection research, the method can follow immune-cell migration toward infected tissues and examine contacts with pathogens or infected cells. These observations help researchers investigate how immune cells participate in inflammation or pathogen clearance. Tracking behavior over time adds a spatial and dynamic perspective to studies of host-pathogen interactions and immune function.
Fluorescent cell tracking can generate quantitative time-lapse data rather than only qualitative visual observations. Researchers can use these records to evaluate cell location, movement, survival, and interactions, then relate those measurements to disease progression or treatment responses. In infection studies, the resulting data can support interpretation of immune function, inflammation, pathogen clearance, and host-pathogen interactions.