Peripheral Blood Lymphocytes
Peripheral Blood Lymphocytes - Related Videos
09:47
Generation of Human Alloantigen-specific T Cells from Peripheral Blood
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Cited by 2 •
2014
This article describes a method for the generation and propagation of human T cell clones that specifically respond to a defined alloantigen. This protocol can be adapted for cloning human T cells specific for a variety of peptide-MHC ligands.
13:08
Expansion of Human Peripheral Blood γδ T Cells using Zoledronate
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Cited by 87 •
2011
A method to expand γδ T cells from peripheral blood mononuclear cells (PBMC) is described. PBMC-derived γδ T cells are stimulated and expanded using zoledronate and interleukin-2 (IL-2). Large scale expansion of γδ T cells can be applied to autologous cellular immunotherapy of cancer.
03:14
Ex Vivo Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells
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2025
This video demonstrates a technique for the expansion of natural killer (NK) cells directly from peripheral blood mononuclear cells (PBMCs) using a feeder cell line. PBMCs are co-cultured with genetically modified lymphoblastoid feeder cells expressing membrane-bound interleukin-21 (mIL-21), and the culture is supplemented with the cytokines interleukin-2 (IL-2) and interleukin-15 (IL-15). Together, this cocktail of ligands leads to the prolonged expansion of NK cells with high purity.
09:00
Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier
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Cited by 6 •
2017
Here, we describe a human blood-brain barrier model enabling to investigate lymphocyte transmigration into the central nervous system in vitro.
02:45
Isolation of Peripheral Blood Mononuclear Cells from Human Buffy Coats
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2025
In this video, we demonstrate the isolation of peripheral blood mononuclear cells, PBMCs, containing monocytes and lymphocytes from a human buffy coat by density gradient centrifugation. The isolated PBMCs can be used for further analysis.
FAQs
Antigen receptors give T and B cells the capacity to recognize specific antigens, linking detection to distinct adaptive responses. Following activation, lymphocytes may release cytokines, undergo clonal expansion, or support antibody production, depending on the response involved. These outcomes help investigators distinguish cellular and antibody-related immune activity during infection studies.
Natural killer cells contribute to innate defense by detecting and destroying stressed or infected cells. T and B lymphocytes instead use antigen recognition as part of adaptive responses. Comparing these populations allows researchers to examine whether an immune response emphasizes rapid cytotoxic activity, antigen-specific cellular activity, or antibody production.
These measurements provide complementary views of immune status. Abundance indicates how many lymphocytes are present, phenotype describes their observable cellular characteristics, and activity reflects how they respond in experimental analyses. Together, the measures can help characterize pathogen-specific responses, evaluate vaccine effects, and follow changes associated with disease progression.
A blood sample can be processed with density-gradient separation to isolate peripheral blood lymphocytes before further study. The recovered cells may then undergo flow cytometry, culture, or functional assays. This workflow provides material for examining cellular abundance, phenotype, and activity rather than relying only on measurements from whole blood.
Flow cytometry, culture, and functional assays provide complementary ways to analyze isolated lymphocytes. Flow cytometry can support assessment of cellular phenotype, while culture and functional testing can be used to examine responses under experimental conditions. Using more than one approach helps connect the presence of particular cells with their measured activity.
They are useful when researchers need to characterize immune status or examine responses associated with infection. Measurements of lymphocyte abundance, phenotype, and activity can support studies of pathogen-specific immunity, vaccine effects, and disease progression. Because the cells can be isolated from blood and tested experimentally, investigators can compare immune features across relevant conditions.