High-throughput Compatible

High-throughput compatible methods are assays or experimental workflows designed to process and analyze large numbers of samples efficiently, making them valuable for scalable immunology and infection research. They typically use miniaturized, parallel reactions in multiwell plates, automated liquid handling, standardized incubation and detection steps, and computational analysis to generate comparable results across many conditions. These workflows support systematic measurement of immune-cell activity, pathogen-host interactions, antibody responses, biomarkers, and potential therapeutic effects. By increasing experimental scale while conserving samples and reagents, high-throughput approaches can accelerate screening, reveal patterns across complex immune responses, and improve the reproducibility of infectious disease studies.

High-throughput Compatible - Related Videos

Research

JoVE Journal - Immunology and Infection

A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis

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Cited by 1 •

2017

New models and assays that would improve the early drug development process for next-generation anti-tuberculosis drugs are highly desirable. Here, we describe a quick, inexpensive, and BSL-2 compatible assay to evaluate drug efficacy against Mycobacterium tuberculosis that can be easily adapted for high-throughput screening.

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators

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Cited by 4 •

2013

The botulinum neurotoxin type A light chain (BoNT/A LC) is a metalloprotease that enters motor neurons, cleaves its substrate SNAP-25, and disrupts neurotransmission, thereby resulting in flaccid paralysis. Utilizing a high-throughput-compatible FRET-based assay, large libraries of small molecules can be screened for their impact on BoNT/A LC enzymatic activity.

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

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2015

Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.

Research

JoVE Journal - Biochemistry
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High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

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Cited by 16 •

2023

This protocol details high-throughput crystallization screening, ranging from the 1,536 microassay plate preparation to the end of a 6 week experimental time window. Details are included about the sample setup, the imaging obtained, and how users can perform analyses using an artificial intelligence-enabled graphical user interface to quickly and efficiently identify macromolecular crystallization conditions.

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

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2023

This protocol provides a rapid method for determining pollen compatibility and incompatibility in citrus cultivars.

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