Antibody Potency Assay
Antibody Potency Assay - Related Videos
08:06
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
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Cited by 1 •
2016
Peptide adsorption to plasticware during traditional tip-based serial dilutions can significantly impact potency determination and confound the understanding of structure-activity relationships used for lead identification and lead optimization phases of drug discovery. Here methods for automated acoustic non-contact serial dilution of peptide samples are described.
16:07
Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method
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Cited by 1 •
2017
A protocol for the determination of the relative anti-apoptotic activity of an anti-TNFα mAb using a neutralization mechanism with WEHI 164 cells is presented here. This protocol is useful for comparing the neutralization strength of different molecules with the same biological functionality.
03:11
A Real-Time In Vitro Cytolysis Assay to Evaluate the Potency of CAR T Cells
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2025
This video demonstrates a method for monitoring CAR T cell activity against cancer cells through real-time electrical impedance measurements. Tracking changes in impedance assesses the effectiveness of CAR T cells in inducing cancer cell cytolysis, providing insights into their cytotoxic capabilities.
10:22
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
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Cited by 5 •
2021
Here we present the mediator release assay, using a rat basophilic leukemia cell line transfected with the human IgE receptor, to simulate the degranulation of effector cells typically observed in type 1 allergic reactions. This method investigates the biological activity of allergens in a highly sensitive, reproducible, and tailorable manner.
05:22
A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses
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2025
Multiplex fluorescence immunoassays are becoming a reference for serology testing. Their application, however, is limited by the complexity and time-consuming bead-analyte coupling processes. This report presents a semi-automated, reproducible multiplex microsphere bead coupling protocol that is comparable to the standard method while considerably reducing time to results.
FAQs
Serial antibody dilutions reveal how the measured biological effect changes as antibody concentration changes. The resulting response pattern allows investigators to identify the concentration range in which inhibition, neutralization, or immune activation can be quantified and to calculate relative potency. This concentration-response relationship provides more information about functional performance than simply recording whether an antibody has any activity.
The assay's target system determines what antibody activity is being tested. A pathogen or antigen can support measurements of neutralization or inhibition, whereas an antigen-expressing cell system can be used to measure a cellular or immune-response outcome. Selecting among these systems connects the readout to the intended biological effect and helps distinguish different types of antibody performance.
Neutralization, infection inhibition, and immune activation are not interchangeable readouts. Neutralization or inhibition focuses on limiting a target-associated process, while activation measures whether antibody exposure stimulates an immune response. Consequently, two antibodies may be compared meaningfully only when the assay uses the same relevant functional outcome and target context.
Relative potency places an antibody's functional result in a comparative framework rather than treating activity as an isolated observation. Serial dilutions are applied to the antibody being evaluated, and the measured outcome is used to compare how strongly it produces the intended effect. This approach supports comparisons among antibody candidates and assessment of consistency across antibody-based products.
A typical workflow begins by preparing serial antibody dilutions, exposing the target antigen, pathogen, or antigen-expressing cells to those dilutions, and measuring the selected functional outcome. The resulting measurements support calculation of relative potency. The exact readout depends on whether the study is designed to assess neutralization, infection inhibition, or activation of an immune response.
In infection research, the method can compare candidate antibodies according to their ability to neutralize or inhibit a pathogen-associated process. In vaccine development, it helps characterize neutralizing activity, while therapeutic development uses it to evaluate functional performance. These applications connect a quantitative laboratory result with the intended protective or treatment-related activity.
For antibody-based products, potency testing contributes to quality control by checking whether functional performance remains consistent. Rather than relying only on the presence of antibody, the assay measures whether the product produces its intended biological effect under the selected test conditions. Comparable potency results can therefore support product characterization and consistency assessment during development.
Within immunology, an activation-based readout can show whether antibody exposure produces an immune response, whereas infection-focused readouts examine neutralization or inhibition. This distinction matters when interpreting protection-related activity, because the selected outcome should match the biological question. The same general assay format can therefore address different immunological and infection-related questions.