In Vivo Fluorescence Imaging
In Vivo Fluorescence Imaging - Related Videos
06:46
Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
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Cited by 4 •
2017
This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation.
11:51
Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
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Cited by 9 •
2009
Mesoscopic fluorescence tomography operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The technique is based on multi-projection illumination and a photon transport description. We demonstrate in-vivo whole-body 3D visualization of the morphogenesis of GFP-expressing wing imaginal discs in Drosophila melanogaster.
09:43
In vivo Near Infrared Fluorescence (NIRF) Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis
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Cited by 32 •
2011
We detail a new near-infrared fluorescence (NIRF) catheter for 2-dimensional intravascular molecular imaging of plaque biology in vivo. The NIRF catheter can visualize key biological processes such as inflammation by reporting on the presence of plaque-avid activatable and targeted NIR fluorochromes. The catheter utilizes clinical engineering and power requirements and is targeted for application in human coronary arteries. The following research study describes a multimodal imaging strategy...
08:15
Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging
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Cited by 4 •
2009
We describe an in vivo fluorescence imaging protocol to monitor muscle regeneration by GFP-labeled myoblasts after transplantation into skeletal muscles of both healthy and dystrophic mice. This protocol can be adapted to study muscle regeneration by transplantation of other types of cells and in other muscular conditions as well.
03:10
In Vivo Fluorescence Imaging to Localize Antibodies in a Mouse Tumor Xenograft Model
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2025
The video demonstrates a fluorescence imaging method for in vivo antibody localization in a mouse tumor xenograft model. It involves the injection of tumor cells in a mouse to generate solid xenografts beneath the skin. Subsequent injection of fluorescent antibodies confirms specific localization through enhanced fluorescence signals within the tumor xenograft.
FAQs
The method depends on separating excitation from emission. An external light source provides wavelengths that activate a fluorescent protein, dye, or targeted probe, and the biological label then emits light at detectable wavelengths. Capturing that emitted signal allows researchers to associate optical patterns with labeled structures or events, creating spatial information without directly observing the underlying tissue.
Fluorescent proteins, dyes, and targeted probes supply different ways to associate a detectable signal with the biological system. Depending on what is labeled, imaging can follow cells, pathogens, molecular events, or gene activity. This labeling step determines which structure or process contributes to the observed signal and therefore shapes the biological question the experiment can address.
Repeated imaging is valuable because it preserves the temporal sequence of a biological process. Rather than viewing separate animals at isolated endpoints, researchers can follow changes in cell trafficking, infection progression, or treatment response over time. The resulting longitudinal record can show when signals appear, shift, or change, helping connect events that a single time point could miss.
Fluorescence can report more than location alone when the label is linked to a relevant molecular or genetic event. Signals may be used to map pathogen localization, host-pathogen interactions, or gene activity, while cellular labels can indicate movement and recruitment. Interpreting the signal therefore requires tying the fluorescent source to the specific biological target being studied.
A basic imaging workflow begins by introducing or associating a fluorescent protein, dye, or targeted probe with the biological system. The subject is then exposed to the appropriate excitation wavelength, and emitted light is captured for analysis. Repeating acquisition across time converts individual observations into a longitudinal view of trafficking, infection, molecular activity, or response.
In immunology and infection studies, the approach can track where immune cells are recruited and where pathogens localize during disease. It also supports observation of host-pathogen interactions in the same living system. These measurements connect spatial distribution with disease-related dynamics, allowing investigators to examine whether cellular movement and pathogen presence change together over the course of an experiment.
Treatment studies can use serial fluorescence measurements to monitor response as it develops instead of relying only on a terminal measurement. Changes in labeled infection, immune-cell behavior, or another targeted signal can be compared across successive observations. Because the same living organism can be followed longitudinally, the design may reduce the need to sacrifice animals at every experimental time point.