Negative Pressure Maintenance
Negative Pressure Maintenance - Related Videos
11:26
Assessing Cerebral Autoregulation via Oscillatory Lower Body Negative Pressure and Projection Pursuit Regression
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Cited by 7 •
2014
Cerebral perfusion is maintained across a range of pressures via cerebral autoregulation. However, characterizing autoregulation requires prominent pressure fluctuations at regulated frequencies. The described protocol will show how oscillatory lower body negative pressure can generate pressure fluctuations to provide data for projection pursuit regression for quantification of the autoregulatory curve.
08:46
Drosophila Maintenance
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2023
Drosophila melanogaster, commonly known as fruit flies, are a frequently used model organism for life science research. Although starting a collection of these critters may seem as easy as leaving a banana on your kitchen counter for too long, a productive fly colony in the lab requires careful husbandry and maintenance. This video demonstrates the necessary steps for maintaining a healthy fly stock. The overview begins with the preparation and storage of the yeast and sugar-containing media on...
14:09
Tilt Testing with Combined Lower Body Negative Pressure: a "Gold Standard" for Measuring Orthostatic Tolerance
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Cited by 31 •
2013
We describe a "gold standard" for evaluating orthostatic tolerance (OT) using tilt testing with combined lower body negative pressure (LBNP). This can be combined with non-invasive evaluations of cardiovascular reflex control. Normal and abnormal responses are defined.
14:01
Anesthesia Induction and Maintenance
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN The Guide for the Care and Use of Laboratory Animals ("The Guide") states that pain assessment and alleviation are integral components of the veterinary care of laboratory animals. The definition of anesthesia is the loss of feeling or sensation. It is a dynamic event involving changes in anesthetic depth with respect to an animal's metabolism, surgical stimulation, or variations in the...
10:54
C. elegans Maintenance
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2023
Ceanorhabditis elegans has been, and is still, used to great success as a model organism for studying a variety of developmental, genetic, molecular and even physical phenomena. In order to use C. elegans to its full potential, proper care and attention to the basic maintenance of this powerful organism is essential. In this video you will learn the basic housing and feeding requirements of C. elegans, how to correctly handle and manipulate worms using a worm pick and how to freeze and recover...
FAQs
Negative pressure maintenance depends on an airflow imbalance: exhaust airflow must remain greater than supply airflow. This difference creates a pressure gradient that favors movement from adjacent spaces into the controlled room, rather than outward. Maintaining that direction helps route potentially contaminated air toward designated ventilation or filtration systems, supporting containment where infectious agents may be present.
Continuous monitoring provides evidence that the intended pressure relationship is still operating. Because containment depends on the relative amounts of exhaust and supply air, a change in either can weaken inward airflow. Monitoring therefore serves as a performance check, helping facilities verify that the engineering control continues to direct air toward the designated ventilation or filtration pathway.
Negative pressure maintenance is an engineering control, not a substitute for other protective measures. Its value increases when paired with appropriate personal protective equipment, cleaning, and procedural controls. These measures address exposure through complementary routes: airflow management limits pathogen release, while protective and operational practices strengthen overall biosafety for workers, researchers, and nearby occupants.
Establish exhaust airflow greater than supply airflow, direct the resulting airflow toward the designated ventilation or filtration system, and continuously monitor performance. This sequence links the pressure differential to a defined containment pathway and allows the facility to verify that the control remains effective during use. The same principles apply across healthcare and laboratory settings.
The approach is particularly relevant to isolation rooms, microbiology laboratories, and other facilities that handle infectious agents. In these environments, controlling the direction of room air can reduce the chance that potentially contaminated air reaches surrounding areas. It therefore supports both healthcare infection control and laboratory biosafety when airborne contamination is a concern.
Work involving infectious agents can create a need to prevent pathogen-containing air from moving into adjacent spaces. By supporting inward airflow and routing exhaust through designated ventilation or filtration systems, negative pressure maintenance helps protect healthcare workers, researchers, and nearby occupants. It therefore contributes an environmental layer of protection within a broader biosafety strategy.