Supernatant Discard
Supernatant Discard - Related Videos
01:49
An Assay To Evaluate the Anticancer Effects of Lactobacillus Cell-Free Supernatant
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2025
This video demonstrates an in vitro assay to study the anticancer effects of Lactobacillus cell-free supernatant (LCFS). Upon exposing colorectal cancer spheroids to varying doses of LCFS, the interaction with the bacterial metabolites in LCFS induces apoptosis in the cancer cells, disrupting the spheroid structure and leading to cell death in a dose-dependent manner.
07:29
Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue
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Cited by 19 •
2011
A simple and reliable method on isolation and culture of neural stem cells from discarded human fetal cortical tissue is described. Cultures derived from known human neurological disorders can be used for characterization of pathological cellular and molecular processes, as well as provide a platform to assess pharmacological efficacy.
03:40
Heparin Affinity Chromatography-Based Baculovirus Purification: A Technique to Isolate Baculovirus From Insect Cell Supernatant
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2025
In this video, we demonstrate the technique to isolate baculovirus from an insect cell supernatant using heparin affinity chromatography. The isolation is facilitated by the affinity between the heparin in the column to the baculovirus envelope glycoprotein.
06:07
Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
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Cited by 15 •
2020
Here methods are presented to understand anti-cancer effects of Lactobacillus cell-free supernatant (LCFS). Colorectal cancer cell lines show cell deaths when treated with LCFS in 3D cultures. The process of generating spheroids can be optimized depending on the scaffold and the analysis methods presented are useful for evaluating the involved signaling pathways.
08:36
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
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Cited by 7 •
2018
Real-time quantitative polymerase chain reaction analysis combined with reverse transcription (RT-qPCR) has been widely used to measure the level of RNA virus infections. Here we present a direct RT-qPCR assay, which does not require an RNA purification step, developed for the quantification of several RNA viruses, including dengue virus.
FAQs
Centrifugation concentrates components according to differences in size and density. Materials that settle into the pellet become the retained fraction, while the surrounding liquid remains above it. The discard step therefore depends on successful prior separation: if the target material does not form a sufficiently settled pellet, removing the liquid may also reduce sample recovery and affect later analyses.
The pellet contains the concentrated cells, microorganisms, or other particulate material being retained. Disturbing it can resuspend material into the liquid layer, causing loss during removal and potentially increasing unwanted carryover. Careful handling helps preserve recovery, reduce contamination, and maintain more consistent sample composition for subsequent culture, staining, or molecular analysis.
Aspiration provides controlled removal of the supernatant while allowing the operator to avoid the settled material at the tube bottom. Its accuracy affects how much liquid is removed and whether the pellet remains intact. Consistent aspiration is especially important when comparable sample handling is needed across immunology or infection experiments.
First, centrifugation is used to settle the target particulate material into a pellet. The tube is then handled carefully so the liquid layer remains distinct from the settled fraction. The supernatant is aspirated or otherwise removed without disturbing the pellet, leaving the concentrated material available for the next preparation or analysis step.
In these fields, the step supports several workflows, including cell washing, pathogen processing, and immune-cell isolation. It can also prepare concentrated material for culture, staining, or molecular analysis. Its value lies in separating the retained biological material from the surrounding liquid so that downstream procedures begin with a more suitable sample fraction.
The amount of supernatant removed and the degree of pellet disturbance influence what remains in the tube. Consistent handling helps preserve the intended concentrated sample, limit unwanted contamination, and improve recovery. These effects matter when samples proceed to culture, staining, or molecular analysis, because variable preparation can contribute to differences between experimental results.