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JoVE Journal
Immunology and Infection

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Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
 

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Article DOI: 10.3791/58407-v 08:36 min November 1st, 2018
November 1st, 2018

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Summary

Real-time quantitative polymerase chain reaction analysis combined with reverse transcription (RT-qPCR) has been widely used to measure the level of RNA virus infections. Here we present a direct RT-qPCR assay, which does not require an RNA purification step, developed for the quantification of several RNA viruses, including dengue virus.

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Keywords: Dengue Virus RNA Quantification Real-time PCR RNA Standard In Vitro Transcription RNA Purification Viral RNA Detection Virus Research Drug Screening Clinical Diagnosis
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