Tcr Engagement
Tcr Engagement - Related Videos
19:05
Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
0 Views •
Cited by 9 •
2015
This manuscript describes how to conduct (single molecule) Förster Resonance Energy Transfer (FRET)- based assays to measure the binding dynamics between T-cell antigen receptor (TCR) and antigenic peptide-loaded MHC molecules as they occur within the immunological synapse of a T-cell in contact with a functionalized planar supported lipid bilayer.
16:10
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
0 Views •
Cited by 13 •
2012
The compartmentalization of proteins either within the plasma membrane or into intracellular locations is one regulatory mechanism that can greatly influence signaling outcomes; hence, to understand signaling it is important to study the spatial and temporal behavior of the proteins involved. We describe here a TIRF microscopy based system to study signal transduction in T cells, but is broadly applicable.
11:21
Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
0 Views •
Cited by 3 •
2017
The current protocol combines single cell paired human TCR alpha and beta chain sequencing with streamlined generation of retroviral vectors compatible with in vitro and in vivo TCR expression.
14:02
Measuring Engagement of Spectators of Social Digital Games
0 Views •
2021
We propose a methodology that enables measuring engagement of spectators in a social digital game combining physiological and self-reported data. As this digital game involves a group of freely moving people, the experience is filmed using a synchronizing technique that links physiological data with events in the game.
06:42
Identifying Kinase Inhibitors that Modulate the Thymocyte Response to Strong TCR Signals
0 Views •
2025
In this video, we describe a method to identify the small-molecule kinase inhibitors that modulate the apoptosis of self-reactive CD4+CD8+ double-positive immature thymocytes. Apoptosis is induced in the double-positive thymocytes by activating them with anti-CD3- and anti-CD28-coated magnetic beads; this is followed by a small-molecule inhibitor treatment and flow cytometry analysis to detect if the inhibitors modulate the apoptotic marker expression.
FAQs
Their proximity enables associated kinases to phosphorylate signaling components, creating a cascade rather than a single activation event. This cascade regulates calcium signaling and changes in gene expression, linking recognition at the cell surface to functional T-cell responses. The resulting molecular sequence helps determine whether a T cell proceeds toward activation and broader adaptive immune activity.
CD4 and CD8 strengthen and refine the interaction between the T-cell receptor and the peptide-MHC complex. Their contribution helps organize signaling components at the contact site, supporting efficient communication between recognition and intracellular responses. Examining these co-receptors is therefore important when interpreting how T cells respond to different antigen-presenting contexts during immune surveillance or infection.
The immunological synapse concentrates the TCR complex, CD3 signaling proteins, co-receptors, and accessory molecules at the interface between a T cell and an interacting cell. This organization supports coordinated signal transmission instead of isolated molecular contacts. Its formation helps connect antigen recognition with calcium signaling, gene expression, cytokine production, and other outcomes of T-cell activation.
Studies can assess phosphorylation cascades, calcium signaling, changes in gene expression, cytokine production, clonal expansion, and the development of immune memory. These readouts represent different stages or consequences of the response, from early intracellular signaling to longer-term adaptive immunity. Comparing them helps investigators determine how effectively recognition is translated into T-cell function.
During infection, analyzing this process clarifies how T cells recognize pathogen-derived peptides presented by MHC molecules and how that recognition develops into adaptive responses. Researchers can connect receptor signaling with cytokine production, clonal expansion, and immune memory. The same framework also helps investigate dysfunction during chronic infection, where effective T-cell responses may not be maintained.
TCR engagement provides a framework for understanding how antigen recognition can initiate and shape protective T-cell responses. In vaccine research, the relevant outcomes include clonal expansion, cytokine production, and immune memory. In immunotherapy research, examining receptor signaling and accessory interactions can help explain or guide efforts to influence T-cell activation and function.