Mouse Subcutaneous Model
Mouse Subcutaneous Model - Related Videos
04:13
An Adjuvant Therapy in a Mouse Model with an Incompletely Resected Subcutaneous Tumor
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2025
This video illustrates testing of adjuvant or additional therapy in a mouse model with incompletely resected subcutaneous tumors. The partially tumor-resected mouse is treated with an antibody targeting the anti-cytotoxic protein of T regulatory cells, potentially reducing tumor regrowth and suggesting effective adjuvant therapy.
03:44
A Unique Mouse Model for Quantitative Assessment of Biofilm Formation on Surgical Implants in Subcutaneous Abscess
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Cited by 1 •
2025
This protocol describes a unique experimental model of implant-related infections that enables the simultaneous incubation of two implants with bacteria under identical conditions within a single mouse. It also allows precise assessment of biofilm formation on implant surfaces using optimized comparative analytical methods, demonstrating advanced techniques for evaluating biomaterials' antimicrobial properties.
08:31
Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging
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Cited by 26 •
2015
We present an experimental procedure of Candida albicans biofilm development in a mouse subcutaneous model. Fungal biofilms were quantified by determining the number of colony forming units and by a non-invasive bioluminescence imaging, where the amount of light that is produced corresponds with the number of viable cells.
03:53
Subcutaneous Dihydrotestosterone (DHT) Treatment: Mouse Model of Polycystic Ovary Syndrome (PCOS)
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2023
This article describes a way to prepare and insert dihydrotestosterone (DHT) pellets to model Polycystic Ovary Syndrome (PCOS) in mice. The example protocol demonstrates the procedure in mice used for reproductive and metabolic studies.
07:49
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
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Cited by 15 •
2015
This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.
FAQs
The tissue beneath the skin provides a localized setting in which introduced cells, tissues, pathogens, or test materials can interact with host tissue. This placement allows investigators to examine immune-cell recruitment, inflammation, and lesion development near the exposure site. Localized responses can help distinguish tissue changes associated with the experimental material from broader observations elsewhere in the animal.
The type of material introduced and the resulting local tissue response are central variables. Depending on the study, investigators may focus on pathogen behavior, implanted biological materials, inflammatory changes, immune-cell recruitment, or lesion development. Measuring several response types together provides a broader interpretation than relying on a single visible or histological feature.
It creates a controlled location for examining how a pathogen and host tissue respond to one another in a living animal. Investigators can follow local inflammation, immune-cell recruitment, and lesion development while assessing changes over time. These observations support analysis of inflammatory mechanisms and can help compare how different experimental groups respond to the same infectious challenge.
Assessment can combine physical, visual, and histological measurements. Physical and visual observations provide information about changes that can be followed at the exposure site, whereas histological analysis examines tissue changes in greater detail. Using these complementary measurements helps connect visible lesion development with underlying inflammation or cellular recruitment and supports comparisons among experimental groups.
A typical workflow begins by introducing the selected cells, tissues, pathogens, or test materials beneath the skin, followed by observation of the local response. Investigators then monitor tissue changes, inflammation, immune-cell recruitment, lesion development, or growth using appropriate physical, visual, and histological assessments. Repeated monitoring allows responses to be evaluated over time rather than at only one endpoint.
The model is useful when investigators need to examine how a vaccine or therapeutic response affects localized infection, inflammation, or tissue change in a living animal. Experimental groups can be compared through visible, physical, and histological outcomes. This design helps relate treatment exposure to changes in host-pathogen interactions or inflammatory mechanisms at the subcutaneous site.
It can show how implanted biological materials behave within living tissue and whether they are associated with local tissue changes, inflammation, or immune-cell recruitment. Because the site is accessible for observation, investigators can follow these responses over time and compare materials or experimental groups. Histological measurements add tissue-level evidence to physical and visual findings.