Cell Suspension Injection

Cell suspension injection is a medical delivery technique in which living cells are dispersed in a liquid carrier and introduced into a target tissue or anatomical site, enabling cell-based treatment. Before administration, cells are maintained as a uniform suspension and delivered through a syringe or catheter; injection conditions must preserve cell viability while limiting aggregation and tissue injury. In regenerative medicine and clinical research, this approach is used to deliver therapeutic cells for local repair, immune modulation, or replacement of damaged cell populations. Its effectiveness depends on cell identity, dose, distribution, survival, and integration within the recipient tissue.

Cell Suspension Injection - Related Videos

Research

JoVE Journal - Medicine

Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension

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Cited by 32 •

2015

Here we present a community accepted protocol in multimedia format for subretinally injecting a bolus of RPE cells in rats and mice. This approach can be used for determining rescue potentials, safety profiles, and survival capacities of grafted RPE cells upon implantation in animal models of retinal degeneration.

Bacterial Endophthalmitis Mouse Model: A Method for Generating Bacterial Endophthalmitis via Intravitreal Injection of Bacterial Suspension into Eye of Mouse

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2025

This video describes a method for generating a mouse model that mimics bacterial endophthalmitis – an intraocular infection resulting from a bacterial invasion into the eye’s vitreous cavity – via intravitreal injection of a bacterial suspension into the eye. This mouse model helps study the disease mechanism and evaluate therapeutics for it.

Research

JoVE Journal - Biology
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Generation of Single-Cell Suspensions from Mouse Neural Tissue

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Cited by 16 •

2009

Dissociating cells from specific tissue types requires specific parameters for tissue aggitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS Dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on nerual tissue is explained.

Stereotaxic Transplantation of Neural Cell Suspension into a Mouse with Traumatic Brain Injury

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2025

Source: Furmanski, O., et al. Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells. J. Vis. Exp. (2019)In this video, human-induced pluripotent stem cell-derived neural cells are stereotaxically injected into the gray matter-white matter border of the deep cortex in a mouse model of traumatic brain injury. The transplanted cells integrate into the tissue, promoting functional recovery and circuit...

Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions

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Cited by 40 •

2013

Vacuolar and cytosolic pH can be measured in live yeast (S. cerevisiae) cells using ratiometric fluorescent dyes localized to specific cellular compartments. We describe procedures for measuring vacuolar pH with BCECF-AM, which localizes to the vacuole in yeast, and cytosolic pH with a cytosolic ratiometric pH-sensitive GFP (yeast pHluorin).

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